文献类型：期刊文献

中文题名：原花青素对缺血-再灌注损伤后大鼠视网膜Bcl-2和Bax表达的影响

英文题名：Influence of proanthocyanidins on expression of Bcl-2 and Bax after ischemia-reperfusion injury in rat retina

作者：张花治[1];刘莹[1];白丽君[1];颉瑞萍[2]

第一作者：张花治

机构：[1]甘肃中医学院,兰州730000;[2]甘肃中医学院附属医院眼科

第一机构：甘肃中医药大学

年份：2013

卷号：23

期号：2

起止页码：83

中文期刊名：中国中医眼科杂志

外文期刊名：China Journal of Chinese Ophthalmology

收录：CSTPCD

基金：甘肃省自然科学研究基金计划项目(1010RJZA137)

语种：中文

中文关键词：缺血再灌注;原花青素;Bcl-2;Bax;视网膜

外文关键词：ischemia-reperfusion; procyanidins; Bcl-2; Bax; retina

摘要：目的探讨原花青素(proanthocyanidins,PC)对大鼠视网膜缺血-再灌注(retinal ischemiareperfusion,RIR)损伤抑凋亡基因蛋白Bcl-2和促凋亡基因蛋白Bax表达的影响。方法所有大鼠随机分为正常组、模型组、丹参对照组、PC低、高剂量组,每组5只。于造模前2周PC低、高剂量组分别给予100、300 mg/kg剂量灌胃PC混悬液,丹参对照组予丹参颗粒溶液0.09 g/kg剂量灌胃,正常组及模型组给予等量生理盐水灌胃,均1次/d。造模后各组仍继续按原方案给药。除正常组外,其余每组按再灌注时间的不同分为缺血再灌注6、24、48及72 h组。以免疫组化法检测RIR后大鼠视网膜不同时间点Bcl-2和Bax蛋白的表达。结果各组视网膜神经节细胞层及内丛状层均可见一定的Bcl-2和Bax蛋白表达,其中模型组Bcl-2蛋白表达在6h开始表达,48 h达到高峰,72 h明显下降。Bax蛋白表达变化与Bcl-2相近,但高峰时间在24 h。与其他组比较差异均有统计学意义(P<0.05)。在各个时间点,丹参组和PC低、高剂量组与模型组比较,Bcl-2蛋白表达上升(P<0.05),Bax蛋白表达下降(P<0.05),尤以PC高剂量组与模型组之间的差异最明显(P<0.01)。结论大鼠RIR损伤后Bcl-2和Bax蛋白表达均升高,PC可上调Bcl-2蛋白的表达,下调Bax蛋白的表达,从而对视网膜起到保护作用。
OBJECTIVE To investigate the effect of proanthocyanidins on protein expression of Bcl-2 and Bax in rats with retinal ischemia-reperfusion injury. METHODS All rats were randomly divided into the normal group, model group, danshen group, low and high PC groups with 5 rats in each group. The rats in the low and high PC groups received 100, 300mg/kg PC respectively, the danshen control group received 0.09 g/kg danshen granules solution, the normal group and model groups received equal capacity of saline 2 weeks before molding, once a day for 14 days. After modeling all groups continued to give medicine according to the original plan. In addition to the nor- mal group, the rest groups by reperfusion time were divided into 6, 24, 48 and 72h groups. The protein expressions of Bcl-2 and Bax in retina of rats at different time points after RIR were detected by immunohistochemical method. RESULTS Retinal ganglion cell layer and inner plexiform layer of each group were observed by Bel-2 and Bax protein expression, which the Bcl-2 expression in the model group 6h began to express, peaked 48h, 72h decreased obviously. The expression change of Bax was similar to Bcl-2, but the peak time at the 24h. Compared with the other groups there were statistical significant differences （P〈0.05）. At alltime points, danshen group, low and high PC groups compared with the model group, the expression of Bel-2 increased （P〈0.05）, Bax expression decreased （P〈0.05）. Es pecially between the high PC group and the model group the differences were most obvious （P〈0.01）. CONCKYSION,RThe protein expression of Bcl-2 and Bax increased in rat retina after retinal ischemia-reperfusion injury, and PC could upregulate Bcl-2 expression and downregu- late Bax expression, thus it could protect the retina.