文献类型：期刊文献

中文题名：颊针对类风湿关节炎家兔血浆代谢物表达的影响

英文题名：Effects of Buccal Acupuncture on Expression of Plasma Metabolites Spectrum of Rabbits with Rheumatoid Arthritis

作者：颉旺军[1];蒲瑞生[1];方晓丽[1];刘丁龙[1];权可[1];秦晓光[1];苏成红[1]

第一作者：颉旺军

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

年份：2017

卷号：24

期号：5

起止页码：57

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金(81260559)

语种：中文

中文关键词：颊针;体针;类风湿关节炎;液相色谱-质谱联用;代谢组学;兔

外文关键词：buccal acupuncture; body acupuncture; rheumatoid arthritis; LC-MS; metabolomics; rabbits

摘要：目的观察颊针和体针对类风湿关节炎模型家兔血浆代谢组学的影响,探寻与颊针和体针作用机制有关的代谢物。方法选取大耳白兔16只,采用完全弗氏佐剂诱导类风湿关节炎模型,造模后颊针组取颊针"膝"穴,用13 mm×0.32 mm毫针直刺0.2~0.5 mm,捻转为主,穴位刺激15 s,每日1次。体针组选取"膝眼""足三里",用13 mm×0.32 mm毫针直刺2~3 mm,每隔5 min行针1次,采用捻转针法,100次/min,行针15 s,每日1次。连续治疗5 d后应用UPLC-QTOF/MS检测各组血浆代谢物,采用相似度分析、系统聚类分析(HCA)、主成分分析(PCA)及正交偏最小二乘判别分析(OPLS-DA)进行研究,通过载荷分析确定有意义的代谢物。结果相似度分析、HCA及PCA均表明体针组、颊针组、模型组及正常组血浆样品中内源性代谢物存在显著差异;OPLS-DA能将4组不同处理家兔血浆样品代谢物在三维象限中完全分开,且各自样品均聚集在一起;载荷分析表明,颊针组与模型组、体针组与模型组间19种内源性代谢物差异有统计学意义;颊针组苯丙氨酸和酪氨酸代谢中N-乙酰-L-苯丙氨酸及2-苯基丙酸均显著下调,而体针组则显著上调。结论颊针和体针治疗类风湿关节炎的机理有一定相似性,但也存在差异,N-乙酰-L-苯丙氨酸及2-苯基丙酸是其差异的主要潜在生物标志物。
Objective To observe the effects of buccal acupuncture therapy and body acupuncture therapy on plasma metabolomites of rheumatoid arthritis rabbits model;To search for the metabolites related to the mechanism of buccal acupuncture and body acupuncture.Methods Sixteen rabbits were made into rheumatoid arthritis models by complete freund's adjuvant before the treatment.The buccal acupuncture group was treated by buccal acupuncture on the“knee”acupoint;13mm×0.32mm needle straight0.2-0.5mm,twist the main points of stimulation15s,once a day.The body acupuncture group was treated by body acupuncture on the“Xiyan”and“Zusanli”acupoints;with13mm×0.32mm needle straight2-3mm,every5min each time,twisting needle method,100times/min for15s,once a day.After the continuous treatment for5days,UPLC-QTOF/MS was applied to test metabolite from the plasma sample of all groups.Similarity analysis,hierarchical cluster analysis(HCA),principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA)were applied in this research;meanwhileload analysis method was used to identify the meaningful metabolites.Results The similarity analysis,HCA and PCA all showed the significant difference of endogenous metabolites in plasma samples among the buccal acupuncture group,the body acupuncture,the model group and the blank group.The4groups of different plasma sample metabolite were completely separated in three dimensions by OPLS-DA and all the samples were gathered respectively.The load analysis indicated that there were19kinds of endogenous metabolites which showed the significant differences between buccal acupuncture group and model group,and also happened between the body acupuncture group and the model group;N-cetyl-L-phenylalanine and2-phenyl propionate in metabolism of phenylalanine and tyrosine decreased significantly in the buccal acupuncture group while significantly increased in the body acupuncture group.Conclusion There are relative similarity on the mechanism of treating rheumatoid arthritis between buccal acupuncture and body acupuncture.However,N-acetyl-L-phenylalanine and2-phenyl propionate,as the main potential biomarkers of the difference,indicate that the dissimilarity can be significant.