文献类型：期刊文献

英文题名：JA-dependent ROS modulation by acetoin-rich volatiles from amyloliquefaciens FZB42 triggers stomatal closure and enhances abiotic stress tolerance in Arabidopsis

作者：Yue, Liang[1,2,3];Ye, Ailing[1,2,3];Uwaremwe, Constantine[3,4];Xie, Xiaofan[1,2,3];Turatsinze, Andeole Niyongabo[1,2,3];Jin, Ling[5];Wang, Yun[1,6];Liu, Shaofang[7];Zhang, Yubao[1,2,3];Tran, Lam-Son Phan[8];Liu, Yang[1,2,3];Chen, Gaofeng[9];Wang, Ruoyu[1,2,3]

第一作者：Yue, Liang

通信作者：Wang, RY[1]

机构：[1]Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Ecol Safety & Sustainable Dev Arid L, Lanzhou 730000, Peoples R China;[2]Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Lanzhou Ecoagr Expt Res Stn, Lanzhou 730000, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[4]Chinese Acad Sci, CAS Key Lab Trop Forest Ecol, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China;[5]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[6]Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, Key Lab Desert & Desertificat, Lanzhou 730000, Peoples R China;[7]Jiangxi Sci & Technol Normal Univ, Coll Life Sci, Key Lab Bioproc Engn Jiangxi Prov, Nanchang 330013, Peoples R China;[8]Texas Tech Univ, Inst Genom Crop Abiot Stress Tolerance, Dept Plant & Soil Sci, Lubbock, TX 79409 USA;[9]Gansu Shangnong Biotechnol Co Ltd, Baiyin 730900, Peoples R China

第一机构：Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, State Key Lab Ecol Safety & Sustainable Dev Arid L, Lanzhou 730000, Peoples R China

通信机构：[1]corresponding author), Chinese Acad Sci, Northwest Inst Ecoenvironm & Resources, 320 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2026

卷号：305

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20255219800485);Scopus(收录号：2-s2.0-105025673086);WOS:【SCI-EXPANDED(收录号:WOS:001650639900001)】；

基金：This work was supported by International Partnership Program of Chinese Academy of Sciences (grant number 315GJHZ2024123GC) and the Science and Technology Planning Project of Gansu Province (grant number 23JRRA575) .

语种：英文

外文关键词：PGPR; Volatile Organic Compounds; Reactive Oxygen Species; Jasmonic acid

摘要：Microbial volatile organic compounds (VOCs) are key mediators of plant stress resilience. However, the redoxmediated mechanisms by which VOCs from beneficial bacteria remotely orchestrate aerial plant defenses remain elusive. Here, we demonstrate that VOCs from Bacillus amyloliquefaciens FZB42 reprogram Arabidopsis thaliana redox homeostasis, inducing a primed state that confers broad-spectrum tolerance to subsequent abiotic stresses. This pre-adaptive response is characterized by a dual-phase modulation of reactive oxygen species (ROS): enhancing H2O2 levels and inducing stomatal closure under normal conditions, while suppressing excessive ROS generation under drought. We identified acetoin as the key bioactive VOC, whose effect is abolished in bacterial mutants (Delta alsS, Delta alsD) and rescued by exogenous application. Genetic and transcriptomic evidence reveals that stomatal closure is mediated by a jasmonic acid (JA)-ROS signaling module and requires the ABA pathway component OST1. Moreover, these VOC-induced stomatal adjustments are associated with enhanced FZB42 rhizosphere colonization, which is linked to concurrent changes in root exudate profiles file. Our findings decode how rhizobacteria co-opt ROS signaling via volatiles to systemically prime plant defenses, offering a sustainable strategy for improving crop fitness through rhizosphere engineering.