文献类型：期刊文献

中文题名：百合知母汤下调钙调神经磷酸酶表达从而抑制气道重塑

英文题名：Baihe Zhimu Decoction inhibiting airway remodeling by down-regulateing the expression of calcineurin

作者：安耀荣[1];司晓丽[1];王燕[1];赵明春[1];杨艳[1];朱向东[1]

第一作者：安耀荣

机构：[1]甘肃中医药大学

第一机构：甘肃中医药大学

年份：2018

卷号：33

期号：2

起止页码：715

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：甘肃省自然科学基金项目(No.1310Rq JZA098)~~

语种：中文

中文关键词：哮喘;钙调神经磷酸酶;气道重塑;百合知母汤

外文关键词：Asthma rats; Calineurin; Airway remodeling; Baihe Zhimu Decoction

摘要：目的:观察百合知母汤对钙调神经磷酸酶蛋白(CaN)的表达影响,探讨其抑制哮喘大鼠气道重塑的干预机制。方法:7周龄雄性Wistar大鼠随机分为对照组,模型组,百合知母汤低、中、高剂量组,地塞米松组,每组10只。10%卵白蛋白(OVA)溶液腹腔注射致敏,2%OVA溶液超声雾化吸入诱发哮喘发作,构建哮喘大鼠模型。HE染色法观察肺组织病理学改变;图像分析法检测支气管管壁厚度;生物化学法检测大鼠肺组织CaN活性;免疫组织化学检测CaN蛋白的表达。结果:与对照组比较,模型组支气管管壁结构破坏,大量炎性细胞浸润,支气管的厚度和肺组织CaN活性分别增加了43%及33%(P<0.01)。与模型组比较,药物干预后大鼠气道炎性反应得到不同程度地改善,各治疗组支气管管壁厚度以及肺组织CaN活性显著下降(P<0.05,P<0.01)。大鼠支气管管壁厚度与大鼠肺组织CaN活性呈正相关(P<0.01)。结论:百合知母汤能够减轻气道炎性反应,改变气道重组,与下调CaN的表达有关。
Objective：To observe the effects of Baihe Zhimu Decoction on the expression of calcineurin protein and to explore the mechanism of its intervention on the airway remodeling in asthmatic rats.Methods：Seven-week-old male Wistar rats were randomly divided into control group,model group,Baihe Zhimu Decoction low,medium and high dose groups and dexamethasone group.The rats were sensitized with 10% ovalbuminby intraperitoneal injection,then induced with 2% ovalbumin by ultrasonic atomizing inhalation to establish the asthma model.Pathological changes of lung tissue were observed by HE staining.The thickness of bronchial wall was evaluated by image analysis system.CaN activity in lung tissue was detected by biochemistry method and the expression of CaN protein was detected by immunohistochemistry.Results：Compared with the control group,the bronchial wall structure of the model group was destroyed,and large number of inflammatory cell infiltration,bronchial thickness and lung tissue CaN activity increased by 43%（P〈0.01）,33%（P〈0.01）.Compared with the model group,the airway inflammatory response in rats was improved to some extent after drug intervention.The bronchial wall thickness and the CaN activity of lung tissue in each treatment group were significantly decreased（P〈0.05）.There was a positive correlation between the level of the bronchial and the activation of CaN（P〈0.05）.Conclusion：Baihe Zhimu Decoction can reduce airway inflammatory response,alter airway remodeling,and down-regulate the expression of CaN.