文献类型：期刊文献

中文题名：当归松弛子宫平滑肌活性组分富集片段捕获

英文题名：Capture separated fraction enriched in bioactive constituent of relaxing uterine smooth muscle from Angelicae sinensis root

作者：刘峰林[1,2];任远[1];赵磊[2];吴国泰[1];王瑞琼[1]

第一作者：刘峰林

机构：[1]甘肃中医学院,兰州730000;[2]甘肃省高校中(藏)药化学与质量研究重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2015

卷号：31

期号：3

起止页码：78

中文期刊名：中药药理与临床

外文期刊名：Pharmacology and Clinics of Chinese Materia Medica

收录：北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：甘肃省自然科学基金(1212RZA091);甘肃省高校中(藏)药化学与质量研究省级重点实验室开放基金(ZZY-2012-02)

语种：中文

中文关键词：当归;子宫平滑肌;活性组分;缩宫素

外文关键词：Angelicae sinensis root（ 当归） ;oxytocin;uterine smooth muscle ;bioactive constituent

摘要：目的:捕获当归治疗痛经活性组分富集分离片段。方法:采用硅胶柱色谱,用乙酸乙酯∶石油醚1∶9,乙酸乙酯:石油醚4∶6,乙酸乙酯,甲醇依次洗脱,得到流分1,流分2,流分3,流分4洗脱片段,片段组分差异用HPLC指纹图谱表征;采用离体实验观察分离片段干预缩宫素诱导的子宫平滑肌、正常子宫平滑肌的生物效应。结果:与空白组比较,20mg/L流分1、80mg/L流分2有显著的抑制缩宫素引发的子宫平滑肌收缩;与20mg/L流分1比较,160mg/L流分3或流分4抑制缩宫素诱导的子宫平滑肌收缩差异无显著性;与对照组比较,40mg/L流分1,160mg/L流分2松弛正常子宫平滑肌的作用显著;与10mg/L流分1比较,160mg/L流分3或流分4松弛正常子宫平滑肌作用差异无显著性,显示流分3或流分4直接松弛子宫平滑肌作用微弱。结论:当归中低极性物质是治疗痛经的活性组分,其主要富集在流分1、流分2。
Objective ：To capture separated fraction enriched in bioactive constituent of relief dysmenorrhea. Methods： Chromatographic methods were used to obtain the different separated fraction, the petroleum ether extract was subjected to open column chromatography on normal phase silica gel and respectively eluted with petroleum ether/ethyl acetate （9：1 v/v）, petroleum ether/ethyl acetate （6：4 v/v） , ethyl acetate, and finally eluted with methanol, totally obtained were 4 fractions, which were respectively Fraction!, Fraction2, Fraction3, Fraction4. Their components difference are characterized by HPLC fingerprint. Each fraction was investigated to suppress spontaneous contractions and oxyto- cin induced contractions of rat uterine smooth muscle in vitro. The data is expressed as the mean ＋ SD, Statistical significance of differences among groups was assessed using one-way ANOVA by SPSS11.0. Results：The results show that 20mg/L Fractionl or 80mg/L Fraction2 be- gin to suppress oxytocin induced contractions of rat uterine smooth muscle in vitro, but Fraction3 160mg/L or Fraction3 160mg/L suppression oxytocin induced contractions is equal with Fractionl 20mg/L, no significance of differences between Fraction3 or Fraction4 160mg/L and Fractionl 20mg/L. Fractionl 40mg/L and Fraction2 160mg/L can relax rat uterine smooth muscle of loading 2 g. Fraction3 160mg/Land Fraction3 160mg/L relax rat uterine smooth muscle of loading 2 g is equal with Fractionl 10mg/L, the result shows that Fraction3 and Frac- tion4 have weaker relaxation on rat uterine smooth muscle. Conclusion： Fractionl and Fraction2 are fractions enriched in rehef-dysmenorrhe- al-bioactive-constituents that are low polar components of Angeheae sinensis root.