文献类型：期刊文献

中文题名：敦煌医方瞿麦汤对肾草酸钙结石大鼠核转录因子2-NAD(P)H醌氧化还原酶1通路的影响

英文题名：Effects of Dunhuang medical prescription Qumai Decoction on nuclear transcription factor 2-nad(P)H quinone oxidoreductase 1 pathway in calcium oxalate nephrolithiasis rats

作者：李荣科[1];颜春鲁[1,2];安方玉[1,2];刘永琦[1,2];刘雪松[1];赵文坤[1];杨晓蓉[1]

第一作者：李荣科

机构：[1]甘肃中医药大学,兰州730000;[2]敦煌医学与转化教育部重点实验室,兰州730000

第一机构：甘肃中医药大学

年份：2020

卷号：26

期号：6

起止页码：756

中文期刊名：中国中医基础医学杂志

外文期刊名：JOURNAL OF BASIC CHINESE MEDICINE

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD_E2019_2020】；

基金：敦煌医学与转化省部共建教育部重点实验室开放基金项目(DHYX17-08,DHYX17-09)-敦煌医方加味瞿麦汤颗粒剂的工艺优化与质量研究,基于Nrf2-ARE-NQO1信号通路探讨敦煌医方加味瞿麦汤对肾草酸钙结石的影响;甘肃省高校大学生就业创业能力提升工程项目(大学生创新创业一体化实践平台建设,6-1);甘肃中医药大学教学研究与教学改革项目(ZH-201701)-基于实践能力和科研素养融合培养构建中医院校大学生创新平台的研究与实践。

语种：中文

中文关键词：瞿麦汤;肾结石;氧化应激;Nrf2-NQO1通路

外文关键词：Qu Mai decoction;Nephrolithiasis;Oxidative stress;Nrf2-NQO1 pathway

摘要：目的:研究探讨敦煌医方瞿麦汤对肾草酸钙结石模型鼠Nrf2-NQO1通路的影响。方法:SD雄性大鼠60只按随机数字表法分为空白对照组(等体积蒸馏水)、模型组(等体积蒸馏水)、肾石通颗粒组(5 g/kg·d),敦煌医方瞿麦汤高、中、低剂量组(20、10、5 g/kg)每组各10只。大鼠肾草酸钙结石模型的制备采用1%乙二醇+2%氯化铵的溶液联合灌胃法,造模5 h后给予相应药物干预,持续4周。末次给药24 h后,检测各组大鼠的体质量、肾指数、血清钙(calcium,Ca^2+)、磷(phosphorus,P)含量、肾组织SOD活性、MDA含量、Nrf2 mRNA和QNO1mRNA的表达。结果:与空白对照组比较,模型组大鼠肾指数、血清Ca^2+含量、肾组织MDA的含量均增加,其体质量、肾组织SOD活性、Nrf2mRNA和NQO1mRNA的表达均下降;与模型组比较,敦煌医方瞿麦汤各干预组肾指数、血清Ca^2+含量均下降,体质量和肾组织SOD活性均升高,MDA含量均下降;敦煌医方瞿麦汤中、高剂量组肾组织Nrf2 mRNA和NQO1mRNA表达均升高。结论:敦煌医方瞿麦汤可通过上调Nrf2-NQO1通路相关因子表达,抑制大鼠肾草酸钙结石的形成。
Objective To study the Nrf2-NQO1 pathway effects of Dunhuang Yifang Qu Mai decoction on calcium oxalate nephrolithiasis rats.Method 60 male SD rats were randomly divided into blank control(equal volume distilled water)group,model(equal volume distilled water)group,Shenshitong granules(5 g/kg)group,Dunhuang Yifang Qu Mai decoction high-dose,middle-dose and low-dose(20、10、5 g/kg.d)groups,10 for each group.Except for blank control group,all the groups were given ig 1% ethylene glycol and 2% ammonium chlorid(2% NH4Cl)to establish calcium oxalate nephrolithiasis model.after 5 h of model establishment,corresponding drugs were given ig for 4 weeks.After 24 h of the last treatment,the weight body and the kidney index were monitored,the contents of serum Ca^2+,P were detected,and the levels of kidney SOD,MDA,Nrf2 and NQO1 were determined.Result Compared with those of blank control group,the kidney index,content of serum Ca^2+,MDA content in kidney tissue were increased and the weight body,SOD antivity,Nrf2 and NQO1mRNA expressions were all lowed in model group,with significant differences(P<0.05).Compared with those of model group,the kidney indexes were markedly decreased in Dunhuang Yifang Qu Mai decoction intervention group,the contents of serum Ca^2+ and MDA contents in kidney tissue were both decreased obviously in Dunhuang Yifang Qu Mai decoction intervention group,the weight body and the SOD antivities in kidney tissue were obviously increased in Dunhuang Yifang Qu Mai decoction intervention group,the Nrf2 and NQO1mRNA expressions were both raised sharply in Dunhuang Yifang Qu Mai decoction middle-dose and high-dose group.Conclusion Dunhuang Yifang Qu Mai decoction could effectively inhibit the formation of calcium oxalate stone in rats via increasing the relevent cytokines expressions of Nrf2-NQO1 pathways.