文献类型：期刊文献

中文题名：针刺对大鼠颅脑损伤后血浆中血栓烷B_2和6-酮-前列腺素F1α的影响

英文题名：Effects of acupuncture on thromboxane B_2 and 6-keton- prostaglandin F1 alpha in plasma of rats following craniocerebral injury

作者：赵永烈[1];张侬[2];王玉来[1];陈锦锋[1];王爱成[1];刘强[2]

第一作者：赵永烈

机构：[1]北京中医药大学东方医院神经内科,北京市100029;[2]甘肃中医学院针灸骨伤学院,甘肃省兰州市730000

第一机构：北京中医药大学东方医院神经内科,北京市100029

年份：2005

卷号：9

期号：9

起止页码：120

中文期刊名：中国临床康复

外文期刊名：Chinese Journal of Clinical Rehabilitation

收录：北大核心:【北大核心2004】；CSCD:【CSCD_E2011_2012】；

语种：中文

中文关键词：脑损伤;血栓烷B2;6-酮-前列腺素F1α;针灸疗法

摘要：目的:颅脑损伤后脑血液循环障碍是病情发展的主要病理生理基础之一,在继发性颅脑损伤中起着重要作用。观察针刺对大鼠颅脑损伤后血浆中血栓烷B2(thromboxaneB2,TXB2)、6-酮-前列腺素F1α(6-ketoprostaglandinF1α,6-keto-PGF1α)的影响。方法:实验于2002-05/09在甘肃中医学院中心实验室、兰州医学院第一附属医院内分泌科完成。56只Wistar大鼠随机分为假手术组、模型组(24,72h)、针刺治疗组(24,72h)、药物治疗组(24,72h),每组8只。参照Feeney自由落体法致大鼠左侧颅脑损伤。按预定时间采集静脉血,将标本作相应处理,用放射免疫分析法测定血浆中TXB2,6-keto-PGF1α的含量。结果:①大鼠颅脑损伤后,血浆中TXB2的含量显著升高,且随着病情的发展上升更高,24,72h分别为(3420.71±1153.16),(3701.21±1133.67)ng/L;血浆中6-keto-PGF1α的含量下降,在72h后缓慢恢复,24,72h分别为(18.91±12.19),(21.99±9.45)ng/L;TXB2/6-keto-PGF1α(T/K)值明显升高,在72h缓慢恢复,24,72h分别为(204.28±76.28),(176.07±57.94)。②24h针刺组血浆中TXB2的含量下降,为(2935.32±1166.47)ng/L,6-keto-PGF1α的含量逐渐恢复,为(27.56±14.17)ng/L;T/K值升高,为108.04±41.71,与假手术组比较差异有显著性意义(P<0.01),但低于模型组。
AIM:Disturbance of blood circulation is one of the main foundations of pathology and physiology of the development of illness and plays an important role in secondary craniocerebral injury.To investigate the effects of acupuncture on thromboxane B2(TXB2) and 6- keton- prostaglandin F1 alpha(6- K- PGF1α ) in the plasma of rats following craniocerebral injury.METHODS:The experiment was finished in the Central Laboratory of Gansu College of Traditional Chinese Medicine from May to September 2002.Fifty- six Wistar rats were randomly divided into following groups:the sham operation group,the brain injury model groups(24 and 72 hours),the acupuncture treatment groups(24 and 72 hours) and the drug treatment groups(24 and 72 hours).Eight rats for every group.According to Free- Falling- Body- Crash- Device of Feeney,the brain- injury model was established and craniocerebral injury was induced in the left cerebrum.The venous blood was obtained according the preconcerted time.The collections were treated correspondly.The contents of TXB2 and 6- keto- PGF1α in plasma were assayed applying radioimmunoassay(RIA). RESULTS:① The content of TXB2 in plasma of rats increased markedly following brain injury, with the development of patient's condition, the content of TXB2 increased higher [24 hours:(3 420.71± 1 153.16) ng/L, 72 hours:(3 701.21± 1 133.67) ng/L].The content of 6- keto- PGF1α decreased,and recovered slowly at 72 hours[24 hours:(18.91± 12.19) ng/L,72 hours:(21.99± 9.45) ng/L].The value of T/K markedly decreased,recovered slowly at 72 hours also[24 hours:(204.28± 76.28),72 hours:(176.07± 57.94)].② In the acupuncture treatment group, at 24 hours after injury,the content of TXB2 in plasma of rats decreased[(2 935.3 ± 1 166.47) ng/L] while the content of 6- keto- PGF1α recovered gradually[(27.56± 14.71) ng/L].The value of T/K increased(128.04± 41.71),but lower than the model group,there was significant difference as compared with the model group and sham operation group respectively(P< 0.01).③ In the acupuncture treatment group at 72 hours after injury,the content of TXB2 in plasma of rats decreased[(1 409.48± 675.55) ng/L].There was significant difference as compared with the model group(P< 0.01), but no significant difference as compared with the sham operation group(P >0.05). The content of 6- keto- PGF1α increased[(39.30± 22.65) ng/L].The value of T/K(42.66± 25.47) was higher than that of the sham operation group,but there was no significant difference as compared with the sham operation group(P >0.05). CONCLUSION:The acupuncture treatment can decrease the heightened content of TXB2 significantly following craniocerebral injury and increase the decreased content of 6- keto- PGF1α significantly.Meanwhile,it can recover the disequilibrium value of T/K.It also can amend vascular abnormal contracted condition,relief vascular spasm,depress abnormal aggregation of thrombocyte,guarantee cerebral energy supply,promote the repair of injured tissue and inhibit secondary craniocerebral injury.