文献类型：期刊文献

英文题名：Exploring the pathogenesis of type 2 diabetes mellitus intestinal damp-heat syndrome and the therapeutic effect of Gegen Qinlian Decoction from the perspective of exosomal miRNA

作者：He, LiSha[1];Bao, Tingting[2,3];Yang, Yingying[2,3];Wang, Han[3];Gu, Chengjuan[4];Chen, Jia[5];Zhai, Tiangang[2,3];He, Xinhui[6];Wu, Mengyi[7];Zhao, Linhua[3];Tong, Xiaolin[8]

第一作者：He, LiSha

通信作者：Zhao, LH[1];Tong, XL[2]

机构：[1]Chengdu Univ Tradit Chinese Med, Chengdu 611137, Peoples R China;[2]Beijing Univ Chinese Med, Grad Coll, Beijing 100029, Peoples R China;[3]China Acad Chinese Med Sci, Guanganmen Hosp, Dept Endocrinol, Beijing 100053, Peoples R China;[4]Guang Zhou Univ Chinese Med Futian, Shenzhen Hosp, Shenzhen 518000, Peoples R China;[5]Gansu Univ Chinese Med, Lanzhou 730000, Peoples R China;[6]Yunnan Prov Hosp Tradit Chinese Med, Kunming 650000, Yunnan, Peoples R China;[7]Guangdong Prov Hosp Chinese Med, Guangzhou 510120, Peoples R China;[8]Changchun Univ Chinese Med, Endocrinol Dept, Affiliated Hosp, Jilin 130117, Jilin, Peoples R China

第一机构：Chengdu Univ Tradit Chinese Med, Chengdu 611137, Peoples R China

通信机构：[1]corresponding author), China Acad Chinese Med Sci, Guanganmen Hosp, Dept Endocrinol, Beijing 100053, Peoples R China;[2]corresponding author), Changchun Univ Chinese Med, Endocrinol Dept, Affiliated Hosp, Jilin 130117, Jilin, Peoples R China.

年份：2022

卷号：285

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85119498666);WOS:【SCI-EXPANDED(收录号:WOS:000788103600004)】；

基金：Funding This work was supported by the National Natural Science Foundation of China (Grant Nos. 82004242 and 81430097) .

语种：英文

外文关键词：Exosomes; miRNA expression; Gene chip; Type 2 diabetes mellitus; Gegen qinlian decoction; Mechanism

摘要：Ethnopharmacological relevance: Diabetes is a common, complex, chronic metabolic disease. A randomized, double-blind, placebo-parallel controlled clinical study has shown that Gegen Qinlian Decoction (GQD) can reduce glycosylated hemoglobin in type 2 diabetes mellitus (T2DM) intestinal damp-heat syndrome patients in a dose-dependent manner. Aim: To explore the pathogenesis of T2DM intestinal damp-heat syndrome and the therapeutic effect of GQD from the perspective of exosomal microRNA (miRNA). Methods: Eligible patients were selected and treated with GQD for 3 months to evaluate their clinical efficacy. Effective cases were matched with healthy volunteers, and saliva samples were collected. Exosomal miRNA was extracted from saliva and analyzed by chip sequencing. Subsequently, the function of the differential gene and the signal transduction pathway were analyzed using bioinformatics technology. Finally, three target miRNAs were randomly selected from the T2DM group/healthy group, and two target miRNAs in the T2DM before treatment/after treatment group were randomly selected for qPCR verification. Finally, we conducted a correlation analysis of the miRNAs and clinical indicators. The registration number for this research is ChiCTR-IOR15006626. Results: (1) The expression of exosomal miRNA chips showed that there were 14 differentially expressed miRNAs in the T2DM group/healthy group, and 26 differentially expressed miRNAs in the T2DM before treatment/after treatment group. (2) Enrichment results showed that in the T2DM group/healthy group, it was primarily related to cell development, body metabolism, TGF-beta, and ErbB signaling pathways. In the T2DM before treatment/after treatment group, it was mainly related to cellular metabolic regulation processes, and insulin, Wnt, and AMPK signaling pathways. (3) The qPCR verification showed that the expressions of hsa-miR-9-5p, hsa-miR-150-5p, and hsa-miR-216b-5p in the T2DM group was higher (P<0.05). Following GQD treatment, hsa-miR-342-3p and hsa-miR-221-3p were significantly downregulated (P<0.05). (4) hsa-miR-9-5p was positively correlated with BMI (P<0.05), and hsa-miR-150-5p was positively correlated with total cholesterol and triglycerides (P<0.05). The GQD efficacy-related gene hsa-miR-342-3p was positively correlated with the patient's initial blood glucose level (P<0.05), and hsa-miR-221-3p was positively correlated with total cholesterol and triglycerides (P<0.05).Conclusion: The exosomal miRNA expression profile and signaling pathways related to T2DM intestinal damp heat syndrome and the efficacy of GQD were established, which provides an alternative strategy for precision traditional Chinese medicine treatment.