文献类型：期刊文献

英文题名：MOLECULAR SPECTROSCOPY EVIDENCE OF EFFECTIVE CONSTITUENTS IN THE BINDING OF Angelica sinensis TO BOVINE SERUM ALBUMIN: COMPARATIVE BINDING AND THERMODYNAMIC PROFILE

作者：Wang, Zhenzhong[1,2,3];Deng, Xuehua[1,2,3];Hou, Jia[4];Fan, Jing[1,2,3]

第一作者：Wang, Zhenzhong

通信作者：Fan, J[1];Fan, J[2];Fan, J[3]

机构：[1]Hubei Univ Med, Coll Pharm, Shiyan 420000, Peoples R China;[2]Key Lab Wudang Characterist Herbal Study Hubei Pr, Shiyan 420000, Peoples R China;[3]Engn Res Ctr Herbal Utilizat Hubei Prov, Shiyan 420000, Peoples R China;[4]Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China

第一机构：Hubei Univ Med, Coll Pharm, Shiyan 420000, Peoples R China

通信机构：[1]corresponding author), Hubei Univ Med, Coll Pharm, Shiyan 420000, Peoples R China;[2]corresponding author), Key Lab Wudang Characterist Herbal Study Hubei Pr, Shiyan 420000, Peoples R China;[3]corresponding author), Engn Res Ctr Herbal Utilizat Hubei Prov, Shiyan 420000, Peoples R China.

年份：2019

卷号：85

期号：6

起止页码：1064

外文期刊名：JOURNAL OF APPLIED SPECTROSCOPY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000463906800012)】；

基金：This study was supported by the Natural Science Foundation of Hubei Provincial Department of Education (Q20182102), the Scientifi c and Technological Project of Shiyan City of Hubei Province (16Y05, 16Y04), the Initial Project for Post-Graduates of Hubei University of Medicine (2014QDJZR07, 2015QDJZR02), and the Foundation for Innovative Research Team of Hubei University of Medicine (FDFR201602).

语种：英文

外文关键词：Angelica sinensis; fluorescence; bovine serum albumin; ferulic acid; butylidenephthalide; senkyunolide I

摘要：Some bio-effective constituents, such as organic acids and phthalides, from the root of Angelica sinensis can be observed in the blood after oral intake. In this work, two organic acids, ferulic acid and anisic acid, and two phthalides, senkyunolide I and butylidenephthalide, were selected to investigate the interaction with bovine serum albumin systematically, using a fluorescence spectroscopic method under physiological conditions. The Stern-Volmer quenching constant, binding constant, and the corresponding thermodynamic parameters and binding numbers were measured. Synchronous fluorescence and three-dimensional fluorescence spectroscopy were also used. The results provided molecular spectroscopy evidence that some constituents of Angelica sinensis are absorbed and transported into the blood.