文献类型：期刊文献

英文题名：Collagen scaffold combined with human umbilical cord-mesenchymal stem cells transplantation for acute complete spinal cord injury

作者：Deng, Wu-Sheng[1];Ma, Ke[2];Liang, Bing[2];Liu, Xiao-Yin[3];Xu, Hui-You[2];Zhang, Jian[2];Shi, Heng-Yuan[4];Sun, Hong-Tao[2];Chen, Xu-Yi[2];Zhang, Sai[2]

第一作者：Deng, Wu-Sheng

通信作者：Chen, XY[1];Zhang, S[1]

机构：[1]Gansu Univ Chinese Med, Coll Integrated Tradit Chinese & Western Med, Lanzhou, Gansu, Peoples R China;[2]Chinese Peoples Armed Police Force, Characterist Med Ctr, Pingjin Hosp Brain Ctr, Tianjin Key Lab Neurotrauma Repair, Tianjin, Peoples R China;[3]Tianjin Med Univ, Sch Clin Med, Tianjin, Peoples R China;[4]Logist Univ Peoples Armed Police Force, Clin Sch Med, Tianjin, Peoples R China

第一机构：甘肃中医药大学中西医结合学院

通信机构：[1]corresponding author), Chinese Peoples Armed Police Force, Characterist Med Ctr, Pingjin Hosp Brain Ctr, Tianjin Key Lab Neurotrauma Repair, Tianjin, Peoples R China.

年份：2020

卷号：15

期号：9

起止页码：1686

外文期刊名：NEURAL REGENERATION RESEARCH

收录：;Scopus(收录号：2-s2.0-85081410768);WOS:【SCI-EXPANDED(收录号:WOS:000519115200018)】；

基金：This work was supported by the National Natural Science Foundation of China, No. 11932013 (to SZ), 11672332 (to SZ); the National Key Research and Development Plan of China, No. 2016YFC1101500 (to SZ); the Science and Technology Military-Civilian Integration Project of Tianjin of China, No. 18ZXJMTG00260 (to XYC); the Key Project of Science and Technology Support Plan of Tianjin of China, No. 17YFZCSY00620 (to XYC); the Rescue Medical Clinical Center Fund of Tianjin of China, No. 15ZXLCSY00040 (to XYC). The funding sources had no role in study conception and design, data analysis or interpretation, paper writing or deciding to submit this paper for publication.

语种：英文

外文关键词：canine; collagen scaffolds; human; human umbilical cord-mesenchymal stem cells; nerve regeneration; rat; spinal cord injury

摘要：Currently, there is no effective strategy to promote functional recovery after a spinal cord injury. Collagen scaffolds can not only provide support and guidance for axonal regeneration, but can also serve as a bridge for nerve regeneration at the injury site. They can additionally be used as carriers to retain mesenchymal stem cells at the injury site to enhance their effectiveness. Hence, we hypothesized that transplanting human umbilical cord-mesenchymal stem cells on collagen scaffolds would enhance healing following acute complete spinal cord injury. Here, we test this hypothesis through animal studies and a phase I clinical trial. (1) Animal experiments: Models of completely transected spinal cord injury were established in rats and canines by microsurgery. Mesenchymal stem cells derived from neonatal umbilical cord tissue were adsorbed onto collagen scaffolds and surgically implanted at the injury site in rats and canines; the animals were observed after 1 week-6 months. The transplantation resulted in increased motor scores, enhanced amplitude and shortened latency of the motor evoked potential, and reduced injury area as measured by magnetic resonance imaging. (2) Phase I clinical trial: Forty patients with acute complete cervical injuries were enrolled at the Characteristic Medical Center of Chinese People's Armed Police Force and divided into two groups. The treatment group (n = 20) received collagen scaffolds loaded with mesenchymal stem cells derived from neonatal umbilical cord tissues; the control group (n = 20) did not receive the stem-cell loaded collagen implant. All patients were followed for 12 months. In the treatment group, the American Spinal Injury Association scores and activities of daily life scores were increased, bowel and urinary functions were recovered, and residual urine volume was reduced compared with the pre-treatment baseline. Furthermore, magnetic resonance imaging showed that new nerve fiber connections were formed, and diffusion tensor imaging showed that electrophysiological activity was recovered after the treatment. No serious complication was observed during follow-up. In contrast, the neurological functions of the patients in the control group were not improved over the follow-up period. The above data preliminarily demonstrate that the transplantation of human umbilical cord-mesenchymal stem cells on a collagen scaffold can promote the recovery of neurological function after acute spinal cord injury. In the future, these results need to be confirmed in a multicenter, randomized controlled clinical trial with a larger sample size. The clinical trial was approved by the Ethics Committee of the Characteristic Medical Center of Chinese People's Armed Police Force on February 3, 2016 (approval No. PJHEC-2016-A8). All animal experiments were approved by the Ethics Committee of the Characteristic Medical Center of Chinese People's Armed Police Force on May 20, 2015 (approval No. PJHEC-2015-D5).