文献类型：期刊文献

英文题名：Protective effect of astragalus membranaceus and its bioactive compounds against the intestinal inflammation in Drosophila

作者：He, Jianzheng[1,2,3,4];Li, Xu[5];Yang, Shipei[1,2,3];Shi, Yan[3];Dai, Yuting[5];Han, Shuzhen[1,2,3];Wang, Yixuan[5];Lin, Xingyao[4,6];Wei, Benjun[7];Liu, Yongqi[1,2,4,6];Xiu, Minghui[1,2,5,6]

第一作者：何建政;和建政

通信作者：Liu, YQ[1];Xiu, MH[1];Liu, YQ[2];Xiu, MH[2];Liu, YQ[3];Xiu, MH[4];Liu, YQ[5];Xiu, MH[5]

机构：[1]Gansu Univ Chinese Med, Prov level Key Lab Mol Med Major Dis, Lanzhou, Peoples R China;[2]Gansu Univ Chinese Med, Prevent & Treatment Tradit Chinese Med Res Gansu C, Lanzhou, Peoples R China;[3]Gansu Univ Chinese Med, Coll Basic Med, Lanzhou, Peoples R China;[4]Gansu Univ Chinese Med, Key Lab Transfer Dunhuang Med Prov & Ministerial L, Lanzhou, Peoples R China;[5]Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou, Peoples R China;[6]Gansu Univ Chinese Med, Res Ctr Tradit Chinese Med Gansu, Lanzhou, Peoples R China;[7]Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Prov level Key Lab Mol Med Major Dis, Lanzhou, Peoples R China;[2]corresponding author), Gansu Univ Chinese Med, Prevent & Treatment Tradit Chinese Med Res Gansu C, Lanzhou, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Key Lab Transfer Dunhuang Med Prov & Ministerial L, Lanzhou, Peoples R China;[4]corresponding author), Gansu Univ Chinese Med, Coll Publ Hlth, Lanzhou, Peoples R China;[5]corresponding author), Gansu Univ Chinese Med, Res Ctr Tradit Chinese Med Gansu, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85144921336);WOS:【SCI-EXPANDED(收录号:WOS:000903810900001)】；

基金：The authors appreciated the financial support received from the National Natural Science Foundation of China (Nos 82104562, 82004228), Gansu Planning Projects on Science and Technology (No. 20JR10RA332), Scientific Research and Cultivation Project of Basic Disciplines of Integrated Chinese and Western Medicine (No. ZXYJHJC-2020-02). Pharmaceutical Research project of Gansu Provincial Drug Administration (No. 2022GSMPA004).

语种：英文

外文关键词：inflammatory bowel disease; Astragalus membranaceus; intestinal homeostasis; bioactive compounds; Drosophila melanogaster

摘要：Inflammatory bowel disease (IBD) is characterized by chronic and relapsing intestinal inflammation, which currently lacks safe and effective medicines. Astragalus membranaceus (AM), also named Huangqi, is one of the most commonly used fundamental herbs in China. Here, we aimed to investigate mechanism and bioactive compounds of AM on treating sodium dodecyl sulfate (SDS)-induced colitis in Drosophila flies. Our data showed that AM extract (AME) supplementation had no toxic effect in flies, and protected flies against SDS-induced lifespan shortening, intestinal morphological damage, and colon length shortening. Moreover, AME supplementation remarkably rescued SDS-induced intestinal stem cell (ISC) overproliferation and increased reactive oxygen species (ROS) level in the intestine. Mechanistically, AME remarkably rescued the altered expression levels of genes and proteins in c-Jun N-terminal kinase (JNK) and JAK-STAT signaling pathways induced by SDS in gut. Additionally, formononetin, isoliquiritigenin, isorhamnetin, astragaloside I, astragaloside III, vanillic acid, and caffeic acid in AM had protection against SDS-induced inflammatory damage in flies. Taken together, AME could ameliorate the intestinal inflammation partially by suppressing oxidative stress-associated JNK signaling and JAK-STAT signaling pathways. AME may provide a theoretical basis for natural medicine toward treating intestinal inflammatory disease in human.