文献类型：期刊文献

中文题名：磁性碳纳米管亲和色谱材料快速筛选大黄调血脂活性成分

英文题名：Rapid screening of active constituents from Rhei Radix et Rhizoma for regulating blood lipid by cell membrane modified magnetic carbon nanotubes bioaffinity material

作者：武晓玉[1,2,3,4];边惠琴[5];迪丽尼尕尔·阿布都艾尼[6];王洁[6];段文达[1,2,3,4];张启立[3,4];夏鹏飞[3,4];赵磊[1,2,3,4]

第一作者：武晓玉

机构：[1]甘肃中医药大学陇药产业创新研究院;[2]西北中藏药省部共建协同创新中心;[3]甘肃省高校中(藏)药化学与质量研究省级重点实验室;[4]甘肃省道地药材质量标准化研究与推广工程实验室;[5]甘肃中医药大学附属医院;[6]甘肃中医药大学药学院,兰州730000

第一机构：甘肃中医药大学

年份：2025

卷号：37

期号：11

起止页码：2057

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：;北大核心:【北大核心2023】；

基金：甘肃省教育厅高校教师创新基金(2023A-077);国家自然科学基金(82160457)。

语种：中文

中文关键词：大黄;磁性碳纳米管;细胞膜;亲和色谱;快速筛选

外文关键词：Rhei Radix et Rhizoma;magnetic carbon nanotubes;cell membrane;affinity chromatography;rapid screening

摘要：为解决“细胞膜色谱材料负载量小及材料与中药提取液分离繁琐”的问题,本研究制备L02脂肪变性细胞膜修饰磁性碳纳米管亲和色谱材料(cell membrane modified magnetic carbon nanotubes,CM@MCNTs),利用扫描电子显微镜、X射线光电子能谱仪、振动样品磁强计进行表征,结合超高效液相色谱(ultra performance liquid chromatography,UPLC)对大黄调血脂活性成分进行快速筛选,并采取分子对接技术模拟活性成分和相关靶点作用方式。结果显示:CM@MCNTs表面覆盖有细胞膜,且出现Fe 2p(706 eV)、N 1s(398 eV)特征峰,并具有超顺磁性,表明材料制备成功;UPLC分析出13种化学成分被CM@MCNTs特异性吸附,分别为:芦荟大黄素-8-O-β-D-葡萄糖苷、大黄酚-8-O-β-D-葡萄糖苷、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚及6种未知成分。7种已知活性成分和过氧化物酶体增殖物激活受体α(peroxisome proliferators-activated receptorsα,PPARα)结合活性大于羟甲戊二酸酰辅酶A还原酶(3-hydroxy-3-methyl glutaryl coenzyme A reductase,HMGCR),其以氢键结合为主。与细胞膜色谱材料相比,CM@MCNTs的细胞膜负载量提高1.5倍,分离时间缩短为1/20,其具有负载量大、分离速度快的优势。本研究中CM@MCNTs能够实现大黄调血脂活性成分的快速筛选,可为中药复杂体系中降血脂活性成分的高通量筛选提供新思路。
To solve the problems of small loading capacity and complicated separation process of cell membrane chromatography materials,this research prepared L02 steatosis cell membrane modified magnetic carbon nanotube affinity chromatography materials(CM@MCNTs).It was characterized by scanning electron microscopy,X-ray photoelectron spectroscopy and vibrating sample magnetometer.Combined with ultra performance liquid chromatograph(UPLC),it was rapidly screened the active components of Rhei Radix et Rhizoma for regulating blood lipid.Molecular docking technique was used to simulate the action of active components and related targets.The results showed that the surface of CM@MCNTs is covered with cell membrane,and the characteristic peaks of Fe 2p(706 eV)and N 1s(398 eV)appear,and the material is superparamagnetic,indicating that the material has been successfully prepared.UPLC analysis showed that 13 chemical components were specifically adsorbed,respectively:aloe-emodin-8-O-β-D glucopyranoside,chrysophanol-8-O-β-D glucoside,aloe emodin,rhein,emodin,chrysophanol,physcion and six unknown components.The binding activity between the seven known active ingredient and peroxisome proliferators-activated receptorsα(PPARα)was greater than that of 3-hydroxy-3-methyl glutaryl coenzyme A reductase(HMGCR),mainly through hydrogen bonding.Compared with cell membrane chromatography materials,CM@MCNTs has the advantages of large loading capacity and fast separation speed,with the membrane loading capacity increased by 1.5times and separation time shortened to 1/20.CM@MCNTs can realize rapid screening of active ingredients of Rhei Radix et Rhizoma.The research also provide a new idea for the high throughput screening of active ingredients in the complex system of traditional Chinese medicine.