文献类型：期刊文献

中文题名：温通针法对血管性痴呆大鼠行为学及脑组织病理变化的影响

英文题名：Effects of “Warmth-producing Needling for Removing Obstruction” on Hippocampal Morphological Changes and Learning-memory Abilities in Vascular Dementia Rats

作者：杨晓波[1];口锁堂[2];杨晓彬[3];郑魁山[1]

第一作者：杨晓波

机构：[1]甘肃中医学院针灸推拿系,兰州730000;[2]上海中医药大学,上海200032;[3]甘肃中医学院附属医院,兰州730000

第一机构：甘肃中医药大学针灸推拿学院

年份：2007

卷号：32

期号：1

起止页码：29

中文期刊名：针刺研究

外文期刊名：Acupuncture Research

收录：MEDLINE(收录号：17580437);CSTPCD;;Scopus(收录号：2-s2.0-34447520153);CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：血管性痴呆;温通针法;海马;病理形态变化;学习记忆能力

外文关键词：Vascular dementia; ＂Warming-producing Needling for Removing Obstruction＂; Hippocampus; Pathomorphological changes; Learning and memory

摘要：目的:观察温通针法对血管性痴呆大鼠脑组织病理形态及学习记忆功能的影响,为临床治疗血管性痴呆提供实验依据。方法:采用反复夹闭双侧颈总动脉-再灌注造成血管性痴呆大鼠模型。将50只健康Wistar大鼠随机分为空白组、模型组、温通针法组、捻转针法组、药物组,每组10只。温通针法组和捻转针法组分别于“大椎”“百会”“水沟”穴施以温通针法和捻转针法,每穴操作60s,每日1次,共15d。药物组给予尼莫地平悬浊液灌胃。各组大鼠经治疗、跳台试验结束后,断头处死,右侧脑组织切片,HE染色,光镜下观察海马区脑组织细胞病理形态的改变。结果:①行为学测试:模型组跳台试验潜伏期延长,错误次数增多,学习和记忆成绩明显低于空白组(P<0.01);温通针法组、捻转针法组和药物组潜伏期及错误次数明显少于模型组(P<0.01,0.05);药物组和温通针法组之间的差异无显著性统计学意义(P>0.05),温通针法组与捻转针法组比较有显著性统计学差异(P<0.01,0.05)。②形态学结果:空白组可见海马CA1区神经元排列整齐、密集,形态正常,无变性,胶质细胞无增生;模型组可见海马CA1区神经元排列紊乱,出现核固缩,胶质细胞有增生,神经细胞数量减少;药物组海马CA1区有核固缩,但较少,胶质细胞较多,神经细胞数量减少;捻转针法组胶质细胞有增生,但较模型组少,神经细胞数量减少有所改善;温通针法组海马CA1区未见核固缩,胶质细胞数量增生不明显,细胞数量明显增多,CA1区细胞排列较模型组整齐,形态较正常,接近空白组。结论:温通针法能明显减轻脑缺血对大鼠海马CA1区神经元的损伤,改善脑缺血引起的学习记忆障碍。
Objective： To observe the effects of ＂Warmth-producing Needling for Removing Obstruction＂ （WPNRO） on the pathomorphological changes of brain and learning-memory abilities in vascular dementia （VD） rats. Methods： A total of 50 Wistar rats were randomly divided into normal control, model, medication, needle-twirling, and WPNRO groups, with 10 cases in each group. VD model was established by repeated cerebral ischemia-reperfusion via repeated occlusion of the bilateral common carotid arteries under anesthesia （3% embutal 40 mg/kg, i.p. ） . ＂Dazhui＂ （GV 14）, ＂Baihui＂（GV 20） and ＂Shuigou＂（GV 26） were punctured with filiform needles and stimulated with twirling technique and WPNRO technique respectively and continuously for 1 min. Animals of medication group were fed with Nimodipine （0.0108 g/kg）. The treatment was conducted once daily continuously for 15 days. The rats＇ learning and memory results were detected respectively with step-down avoidance test in the first 6 days＇ training and 24 h later. At the end of experiments and after decapitation, the right brain was taken, cut into sections （5 μm） and stained with H ＆ E method for observing structural changes of hippocampus. Results： 1 ） Behavior reactions： compared with control group, the latency for finding the safe platform and the times of error in model group increased significantly, and compared with model group, both latencies and times of error in WPNRO, needle-twirling and medication groups decreased considerably （ P〈0.05, 0.01 ）. In comparison with needle-twirling group, both latencies and times of error in WPNRO group were significantly fewer （P〈0.05, 0.01 ）, and no significant differences were found between WPNRO and medication groups in these two indexes （P〉0.05）. It indicated that acupuncture particularly WPNRO could improve both learning and memory abilities in VD rats. 2） Structural changes of neurons in CA1 region of hippocampus： in normal group, the neurons arranged in order and closely, and were normal in the structure; in model group, the neurons arranged obviously in disorder, had karyopyknosis and hyperplasia in glial cells and decreased in the number. In WPNRO group, no evident cellular karyopyknosis or hyperplasia of glial cells was found, and the structure and number of cells were close to those of normal group. In acupuncture and medication groups, fewer neurons, cellular karyopyknosis and hyperplasia of more glial cells were found. Conclusion： ＂Warming-producing Needling for Removing Obstruction＂ can significantly relieve cerebral ischemia induced disturbance of learning and memory and lessen structural injury of hippocampal neurons in rats.