文献类型：期刊文献

中文题名：秦艽对风湿热痹类风湿关节炎大鼠的保护作用

英文题名：Protective effects of Qinjiao on arthralgia rheumatoidarthritis in model rats

作者：王蓉[1];苏杉[1];孙少伯[2];高慧琴[1]

第一作者：王蓉

机构：[1]甘肃中医药大学药学院中药学科,兰州730000;[2]甘肃中医药大学中西医结合研究所,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2018

卷号：34

期号：1

起止页码：41

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金地区基金资助项目(81360648)

语种：中文

中文关键词：秦艽;药对;类风湿关节炎;踝关节病理学改变;核因子κB

外文关键词：Qinjiao; compatibility; rheumatoid arthritis ; ankle pathological change ; nuclear factor kappa B

摘要：目的研究秦艽对风湿热痹类风湿关节炎(RA)大鼠踝关节核因子κB(NF-κB)的表达及作用机制。方法按照随机数字表法将SD大鼠分为8组,每组10只:正常组、疾病模型组(Ⅱ型胶原)、病证模型组(Ⅱ型胶原+风湿热)、阳性药组、秦艽组、秦艽+威灵仙组、秦艽+桑寄生组、秦艽+防己组。第1天,在大鼠的背部2点和尾根部1点的皮内注射Ⅱ型胶原(CⅡ)乳剂各0.1 mL,致炎;第2天(除正常组和疾病模型组外),用人工气候箱进行风湿热病证模型造模,连续11 d;第12天,在大鼠左后肢足跖皮内注射CⅡ乳剂0.1 mL进行2次免疫作为激发注射,再放入人工气候箱进行风湿热病证模型造模,连续7 d。造模结束后,各给药组按15 mL·kg^(-1)给予对应的药液灌胃,正常组、2种模型组给予等量0.9%NaCl,每天1次,连续21 d。第39天,踝关节取材,用苏木精-伊红(HE)染色技术观察踝关节组织的破坏程度,用免疫组织化学法测定核因子κB(NF-κB)的蛋白表达水平,用免疫印迹法对NF-κB的表达进行半定量分析。结果与NF-κB在正常组的表达量105.08±7.97相比,疾病模型组和病证模型组的NF-κB蛋白表达量分别为170.57±8.93,180.02±6.93,差异均有统计学意义(均P<0.05),且病症模型组高于疾病模型组。各给药组之间比较,秦艽防己组的NF-κB蛋白表达量为112.37±7.34,差异有统计学意义(P<0.05)。NF-κB蛋白相对表达量:与正常组的0.41±0.16比较,疾病模型组和病证模型组的分别为0.78±0.12,1.08±0.11,差异均有统计学意义(均P<0.05);与病证模型组比较,秦艽防己组的NF-κB蛋白相对表达量为0.53±0.04,差异有统计学意义(P<0.05)。结论各秦艽寒热不同配伍可能通过下调NF-κB,对RA起到保护作用,其中平寒相配效果最佳。
Objective To observe effects of Qinjiao（ Gentianae macrophyllae Pall.） in different combinations in wind-damp-heat arthralgia RA model rats and its mechanism. Methods SD rats were randomly divided into eight groups： normal group,disease model group（ type Ⅱcollagen）,syndrome model group（ type Ⅱ collagen ＋ rheumaticfever）,positive drug group,Qinjiao group,Qinjiao ＋ Weilingxian（ Clematis chinensis Osbeck） group,Qinjiao ＋ Sangjisheng（ Taxillus chinensis）group,Qinjiao ＋ Fangji（ Stephaniatetrandra） group,each group had ten rats. In the first day of the experiment,the rats in the other groups were given two points on the back,one point at the end of the tail,shaved and intradermally 0. 1 mL of collagen Ⅱ（ C Ⅱ）. In the second day of the experiment,except for the normal group and disease model group,the other groups were reconstructed with the reconstructed artificial climate box for rheumatism and fever model for 11 days. On the 12 th day of the experiment,0. 1 mL of C Ⅱ emulsion was injected into the left hind paw of the plantar vein to further stimulateand then into the artificial climate box for rheumatoid fever model for 7 days. At the end of the model,the rats in each group were given the corresponding drug by 15 mL·kg^（-1）; the normal group,the disease model group and the syndrome model group were given the same amount of saline once a day for 21 days. The first 39 days of the experiment,takingthe ankle joint,the degree of damage of ankle joint was observed by pathological staining with hematoxylin eosin（ HE）. The expression of nuclear factor kappa B（ NF-κB） protein in ankle jointwas determined by immunohistochemical method. The NF-κB expression was semi-quantitative analysis by Western blot. Results The expression of NF-κB protein in the disease model group and the syndrome model group was 170. 57 ± 8. 93 and 180. 02 ± 6. 93,respectively. Compared with the expression of NF-κB in the normal group of 105. 08 ± 7. 97,the difference was statistically significant（ all P 0. 05）. And the syndrome model group was higher than the disease model group. After administration,compared with others drug groups,the expression of NF-κB protein was 112. 37 ± 7. 34 in Qinjiao ＋ Fangji group statistically significant（ P 0. 05）.Compared with the normal model group（ 0. 41 ± 0. 16）,relative expression of NF-κB protein in the disease model group and the syndrome model group were 0. 78 ± 0. 12,1. 08 ± 0. 11,which was significantly higher than that in normal group,and the difference was statistically significant（ all P 0. 05）. Compared with the syndrome model group,the relative expression of NF-κB protein in Qinjiao ＋ Fangji group was 0. 53 ± 0. 04,and the difference was statistically significant（ P 0. 05）. Conclusion Different compatibility of Qinjiao may down expression of NF-κB to protect on RA,among then the combined effect of mild and cold traditional Chinese medicine（ TCM） is better than the combined effect of mild and warm TCM,the combined effect of mild and mild TCM.