文献类型：期刊文献

中文题名：野菊花提取物对慢性支气管炎大鼠TNF-α及中性粒细胞功能的影响

英文题名：Effect of Extract of Chrysanthemum Indicum on Tumor Necrosis Factor- α Expression and Neutrophil Function in Chronic Bronchitis Rats

作者：苏韫[1];黄勇[1];李娟[1];孙少伯[1];张毅[1];颜春鲁[1];刘永琦[1]

第一作者：苏韫

机构：[1]甘肃中医学院病原生物与免疫学教研室,兰州730000

第一机构：甘肃中医药大学基础医学院（敦煌医学研究所）

年份：2009

卷号：20

期号：4

起止页码：300

中文期刊名：中药新药与临床药理

外文期刊名：Traditional Chinese Drug Research and Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：甘肃省教育厅科研基金资助项目(044-02)

语种：中文

中文关键词：野菊花;慢性支气管炎;肿瘤坏死因子-α;中性粒细胞;吞噬功能;呼吸爆发

外文关键词：Chrysanthemum indicum ; Chronic bronchitis ; Tumor necrosis factor -α ; Neutrophil ; Phagocytosis;Respiratory burst

摘要：目的研究野菊花提取物对慢性支气管炎模型大鼠肿瘤坏死因子(TNF-α)水平及中性粒细胞功能的影响,以阐明其部分疗效机制。方法Wistar大鼠随机分为正常对照组,慢支模型组,喘息灵对照组,野菊花提取物低、中、高剂量组;以气管内注入脂多糖建立慢支大鼠模型,药物干预后,ELISA法测定大鼠血清及支气管肺泡灌洗液TNF-α含量,流式细胞术测定大鼠中性粒细胞(PMN)吞噬能力及呼吸爆发的强度。结果模型组血清及肺泡灌洗液TNF-α含量以及PMN吞噬功能、呼吸爆发强度较正常对照组明显升高,野菊花提取物可显著降低慢支模型大鼠血清及肺泡灌洗液中的TNF-α水平、降低PMN吞噬功能及呼吸爆发强度的异常升高。结论降低慢支大鼠体内TNF-α水平、PMN吞噬功能及呼吸爆发强度可能是野菊花提取物减轻慢性支气管炎炎症效应的机理之一。
Objective To study the effect of the extract of Chrysanthemum Indieum （CI）on the expression of tumor necrosis factor - α （TNF -α） and neutrophil function in chronic bronchitis （CB）rats and to explore the therapeutic mechanism for chronic bronchitis. Methods The extract of CI was prepared. Wistar rats were randomly divided into blank control group, model group, Chuanxiling group, and low - , middle - and high - dose CI extract groups. Rat model of CB was established by intratracheal injection with low -dose lipopolysaccharide. After drug intervention, the expression of TNF -α in rat serum and bronchoalveolar lavage fluid was detected by ELISA method. Phagocytic function of the neutrophil and respiratory burst of the rats were measured by flow cytometry （FCM）. Results Compared with the blank control group, the phagocytic function of the neutrophil, respiratory burst of the rats, and the expression of TNF -α in rat serum and bronchoalveolar lavage fluid was detected by ELISA method. Phagocytic function of the neutrophil and respiratory burst of the rats were measured by flow cytometry （FCM）. Results Compared with the blank control group, the phagocytic function of the neutrophil, respiratory burst of the rats, and the expression of TNF-α in rat serum and bronchoalveolar lavage fluid of the model group were increased significantly. CI extract significantly decreased the abnormal rising of the above indexes. Conclusion Down - Regulation of TNF -α expression, and decrease of the neutrophil phagocytic function and rat respiratory burst may be one of the therapeutic mechanisms of Chrysanthemum Indicum extract for the treatment of CB.