文献类型：期刊文献

英文题名：Oxytropis falcata bunge extract combined with black soybean oil ameliorates DNCB-induced atopic dermatitis-like skin inflammation

作者：Chen, Rupei[1,2];Wu, Yan[1];Wu, Xianwei[2];Bu, Jianhua[1,2];Liu, Meng[1,2];Zhao, Qianya[1,2];Chen, Yan[1,2];Tian, Jitao[1,2];Kai, Jinjin[1,2]

第一作者：Chen, Rupei

通信作者：Wu, Y[1]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Peoples R China;[2]Gansu Prov Hosp, Dept Dermatol, Lanzhou, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：16

外文期刊名：FRONTIERS IN PHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-105009836712);WOS:【SCI-EXPANDED(收录号:WOS:001521804000001)】；

基金：The author(s) declare that financial support was received for the research and/or publication of this article. This work was financially supported by the Education Science and Technology Innovation Project of Gansu Province (Program No. 2023A-085) and the Natural Science Foundation of Gansu Province (Program No. 24JRRA563) and the Gansu province new product engineering laboratory of Traditional Chinese Medicine Open Fund (Program No. ZYXCP-24-07).

语种：英文

外文关键词：Oxytropis falcata bunge; black soybean; atopic dermatitis; HDAC3; NF-kappa B

摘要：Background Oxytropis falcata Bunge (OF) is a traditional Tibetan medicine, while black soybean oil (BSO) is a contemporary treatment used for eczema. Pharmacological studies have indicated that both exhibit strong anti-inflammatory effects. However, the role of OF in atopic dermatitis remains uncertain.Objective To investigate the anti-inflammatory effects and underlying mechanisms of O. falcata Bunge extracts (OFE) and BSO in DNCB-induced atopic dermatitis in mice.Methods Mice were divided into six groups: positive control (1% mometasone furoate), OFE, BSO, OFE + BSO, DNCB, and control. After 20 days of local application of each ointment, therapeutic effects were evaluated. Histopathological examination was performed to assess skin thickness and mast cell infiltration. ELISA was used to quantify proinflammatory cytokines, real-time PCR measured IL-36 mRNA levels, and Western blotting analyzed HDAC3/NF-kappa B and CysLTR1 protein expression.Results All treatments alleviated DNCB-induced atopic dermatitis symptoms, with the combination group showing the most significant improvement in epidermal thickness, mast cell infiltration, and dermatitis severity. In addition, the treatment groups suppressed activation of the HDAC3/NF-kappa B signaling pathway.Conclusion The combination of OFE and BSO can effectively reduce DNCB-induced atopic dermatitis in mice. Its action does not rely on broad immunosuppression or induce skin toxicity and may involve inhibition of proinflammatory cytokine release and downregulation of the HDAC3/NF-kappa B signaling pathway.