文献类型：期刊文献

英文题名：Volatile oil from Acori graminei Rhizoma affected the synaptic plasticity of rats with tic disorders by modulating dopaminergic and glutamatergic systems

作者：Feng, Peng[1];Chen, Yuanhuan[3];Sun, Kexin[3];Wei, Xing[3];Ding, Yanqin[4];Shang, Jing[3];Shi, Zhenggang[3];Xu, Xiaomin[1];Guo, Junxiong[5];Tian, Yongyan[2]

第一作者：Feng, Peng

通信作者：Feng, P[1];Tian, YY[2]

机构：[1]Hexi Univ, Med Coll, Zhangye, Gansu, Peoples R China;[2]Hexi Univ, Silk Rd Tradit Chinese Med Res Ctr, Zhangye, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Clin Coll Chinese Med, Lanzhou, Gansu, Peoples R China;[4]Hexi Univ, Paediat, Zhangye Peoples Hosp, Zhangye, Gansu, Peoples R China;[5]Hexi Univ, Inst Tradit Chinese & Western Med Integrat, Zhangye, Gansu, Peoples R China

第一机构：Hexi Univ, Med Coll, Zhangye, Gansu, Peoples R China

通信机构：[1]corresponding author), Hexi Univ, Med Coll, Zhangye, Gansu, Peoples R China;[2]corresponding author), Hexi Univ, Silk Rd Tradit Chinese Med Res Ctr, Zhangye, Gansu, Peoples R China.

年份：2024

卷号：335

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85201690274);WOS:【SCI-EXPANDED(收录号:WOS:001301406900001)】；

基金：This work was supported by the Science and Technology Plan of Gansu Province (22JR11RG221) ; Doctoral Research Start-up Fund Project of Hexi University (KYQD2022015) ; Research Project of Gansu Provincial Administration of Traditional Chinese Medicine (GZKP-2023-55) ; Hexi University President's Fund for Young Research Projects (QN2023001) ; College Students' Innovation and Entrepreneurship Project of Gansu Province in 2023 (S202310740008, S202310740011) ; Young Doctor Foundation of Higher Education in Gansu Province (2023QB-075) .

语种：英文

外文关键词：Tic disorder; Volatile oil from Acori graminei Rhizoma; Dopamine; Glutamate; Synaptic plasticity

摘要：Ethnopharmacological relevance: Acori graminei Rhizoma is a commonly used traditional Chinese medicine for treating TD, with its main component being calamus volatile oil. Volatile Oil from Acori graminei Rhizoma (VOA)can protect nerve cells and alleviate learning and memory disorders. However, the mechanism of anti-tic of VOA is still unclear. Aim of the study: We aimed to explore the effects of Volatile Oil from Acori Tatarinowii Rhizoma (VOA) on striatal dopaminergic and glutamatergic systems and synaptic plasticity of rats with Tic Disorder (TD), as well as its pharmaceutical mechanism against TD. Materials and methods: This study involved 48 (three-week-old) Sprague Dawley (SD) rats, which were randomly divided into two primary groups: Control (8) and TD (40). Rats in the TD group were injected intraperitoneally with 3,3-iminodipropionitrile (IDPN) to construct the TD rat model. They were divided into five subgroups: Model, Tiapride, VOA-high, VOA-medium, and VOA-low (N = 8). After modeling, VOA was administrated to rats in the VOA groups through gavage (once/day for four consecutive weeks), while rats in the blank control and model groups received normal saline of the same volume. The animals' behavioral changes were reflected using the stereotypic and motor behavior scores. After interferences, patterns of striatal neurons and the density of dendritic spines were investigated using H&E &E and Golgi staining, and the ultrastructure of striatal synapses was examined using Transmission Electron Microscopy (TEM). Furthermore, Ca2+ 2 + content was determined using the Ca2+ 2 + detector, and Dopamine (DA) and Glutamate (GLU) contents in serum and striatum were detected through ELISA. Finally, DRD1, DRD2, AMPAR1, NMPAR1, DAT, VMAT2, CAMKII, and CREB expression in the striatum was detected using Quantitative real-time PCR (qRT-PCR), Western Blotting (WB) and Immunohistochemical (IHC) methods. Results: Compared to rats in the blank control and model groups, rats in the VOA groups showed lower stereotypic behavior scores. Furthermore, rats in the VOA groups exhibited relieved, neuron damage and increased quantities of neuronal dendrites and dendritic spines Additionally, based on TEM images show that, the VOA groups showed a clear synaptic structure and increased amounts of postsynaptic dense substances and synaptic vesicles. The VOA groups also exhibited reduced Ca2+ 2 + contents, and upregulation of DRD1, DRD2, DAT, AMPAR1, and NMPAR1 and downregulation of VMAT-2, CAMKII, and CREB in the striatum. Conclusions: In summary, VOA could influence synaptic plasticity by tuning the dopaminergic and glutamatergic systems, thus relieving TD.