文献类型：期刊文献

中文题名：基于功能性近红外光谱技术观察针刺对创伤后应激障碍大鼠脑部血氧浓度的影响

英文题名：Effect of acupuncture on blood oxygen concentration in brain of rats with post-traumatic stress disorder based on functional near-infrared spectroscopy

作者：张彦峰[1];韩雅迪[1];赵中亭[1];严兴科[1];杨燕萍[2]

第一作者：张彦峰

通信作者：Yan, XK[1]

机构：[1]Gansu University of Chinese Medicine, Lanzhou 730000, China;[2]不详

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2019

卷号：17

期号：1

起止页码：9

中文期刊名：Journal of Acupuncture and Tuina Science

外文期刊名：针灸推拿医学（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2019_2020】；

基金：This work was supported by Regional Science Fund of National Natural Science Foundation of China (No. 81460744).

语种：中文

中文关键词：针刺疗法;穴,百会;穴,神门;穴,内关;穴,太冲;应激障碍,创伤后;光谱,近红外;大鼠

外文关键词：Acupuncture Therapy;Point, Baihui (GV 20);Point, Shenmen (HT 7);Point, Neiguan (PC 6);Point, Taichong (LR 3);Stress Disorders, Post-traumatic;Spectroscopy, Near-infrared;Rats

摘要：目的:基于功能近红外光谱技术(fNIRS)观察针刺对创伤后应激障碍(PTSD)模型大鼠脑部血氧浓度的影响,揭示针刺干预PTSD的脑功能机制。方法:将60只Sprague-Dawley(SD)大鼠随机分为空白组、模型组、抓取组、帕罗西汀组和针刺组,每组12只。除空白组外,其余各组大鼠均以电击幽闭法复制PTSD动物模型,模型复制共7d。于应激造模前1h,各组接受相应干预:空白组和模型组不接受任何干预,抓取组接受抓取固定,帕罗西汀组接受盐酸帕罗西汀溶液灌胃,针刺组接受针刺治疗,6d为1个疗程,连续干预2个疗程,共计12d。造模结束后,各治疗组连续治疗5d后利用fNIRS系统采集并记录各组大鼠相关脑区组织氧合血红蛋白(HbO2)>脱氧血红蛋白(d-Hb)和总血红蛋白(t-Hb)浓度的变化情况,并进行脑功能评估。结果:干预结束后,与空白组比较,模型组和抓取组大鼠HbO2浓度显著升高,d-Hb浓度显著降低,t-Hb浓度显著升高,差异均具有统计学意义(均P<0.01);与模型组比较,抓取组大鼠HbO2.d-Hb和t-Hb浓度均无明显变化(均P>0.05);与抓取组比较,帕罗西汀组和针刺组大鼠HbO2浓度显著降低,d-Hb浓度显著升高,t-Hb浓度显著降低,差异均具有统计学意义(均P<0.05);帕罗西汀组与针刺组的HbO2> d-Hb和t-Hb浓度均无统计学差异(均P>0.05)。结论:针刺对PTSD模型大鼠脑区血氧浓度有良性的调节作用,这可能是针刺干预PTSD的重要脑功能机制。
Objective: To observe the effect of acupuncture on blood oxygen concentration in the brain of rats with post-traumatic stress disorder (PTSD) based on functional near-infrared spectroscopy (fNIRS), thus to reveal the mechanisms of acupuncture in intervening the brain function of PTSD rats. Methods: Sixty Sprague-Dawley (SD) rats were randomly divided into a blank group, a model group, a grasping group, a paroxetine group and an acupuncture group, with 12 rats in each group. Except the blank group, rats in the other groups all received incarceration plus electric shock for 7 d to prepare the PTSD animal model. One hour before the stress model was established, rats in each group received the desig nated in tervention: rats in the blank group and the model group did not receive any intervention;rats in the grasping group received grasping and fixation;rats in the paroxetine group received paroxetine hydrochloride solution by intragastric administration;and rats in the acupuncture group received acupuncture. Six-day treatme nt was a course, with 2 courses of treatme nt con ducted for a total of 12 d. After the modeli ng, rats in each treatment group received intervention for 5 dz and the fNIRS system was used to collect and record the changes in the concentrations of oxygenated hemoglobin (HbO2), deoxygenated hemoglobin (d-Hb) and total hemoglobin (t-Hb) of the involved rat's brain regions, and also to assess the brain function. Results: Compared with the blank group, the con centrati on of HbO2 was significa ntly in creased, the concen trati on of d-Hb was significa ntly decreased, and the concen tration of t-Hb was sign ifica ntly in creased in the model group and the graspi ng group after the in tervention, and the differ en ces were statistically significant (all P<0.01). Compared with the model group, the concentrations of HbO2, d-Hb and t-Hb in the grasping group did not change significantly (all P>0.05). Compared with the grasping group, the concentration of HbC^was significantly decreased, the concentration of d-Hb was significantly in creased, and the concen trati on of t-Hb was sign ifica ntly decreased in the paroxeti ne group and the acup un cture group, and the differences were statistically sigrdficant (all P<0.05). There were no significant differences in the concentrations of HbO2, d-Hb and t-Hb between the paroxetine group and the acupuncture group (all P>0.05). Con elusion: Acup un cture can regulate the blood oxyge n conce ntrati on in the brain of PTSD model rats, which may be an important mechanism of acupuncture in intervening the brain function in PTSD rats.