文献类型：期刊文献

中文题名：当归多糖对AD模型小鼠抗衰老作用研究

英文题名：A Study on the Anti-aging Effects of Angelica Sinensis Polysaccharide on AD Model Mice

作者：安方玉[1];刘雪松[1];张艳霞[1];李雪燕[1];楚惠媛[1];梁健庆[2]

第一作者：安方玉

机构：[1]甘肃中医学院,兰州730000;[2]甘肃中医学院附属医院

第一机构：甘肃中医药大学

年份：2014

卷号：28

期号：11

起止页码：1223

中文期刊名：中国药事

外文期刊名：Chinese Pharmaceutical Affairs

收录：CSTPCD

基金：甘肃省教育厅项目(编号1006B-04)

语种：中文

中文关键词：当归多糖;衰老;学习记忆;NO;NOS;AchE活性;p16蛋白;阿尔茨海默病

外文关键词：Angelica sinensis polysaccharide; aging; learning and memory function; nitric oxide（NO）; nitric oxide synthase（NOS）; acetylcholinesterase activity（AchE）; p16protein; Alzheimer＇s disease（AD）

摘要：目的观察当归多糖对AD模型小鼠学习记忆功能,测定脑组织中NO含量、p16蛋白含量、NOS及AchE活性,探讨当归多糖治疗衰老的作用机制。方法 KM小鼠72只,采用Y型迷宫实验测定小鼠的学习记忆能力,应用生化法测定脑组织NO含量、NOS及AchE活性,ELISA法测定p16蛋白含量。结果当归多糖各剂量组小鼠学习记忆能力明显得到改善,脑组织中NO浓度、NOS活性、AchE活性和p16蛋白含量与对照组比较显著降低。结论当归多糖具有延缓衰老的作用,可能机制是通过抗氧化作用和调控细胞周期实现的。
Objective To investigate the effects of Angelica sinensis polysaccharide（ASP）on AD model mice＇s learning and memory function,measure the content of nitric oxide（NO）,p16 protein,and the activity of nitric oxide synthase（NOS）and AchE in brain tissue,and investigate the therapeutic mechanism of Angelica sinensis polysaccharide on aging.Methods Totally 72 KM mice＇s learning and memory function were measured by Y maze test.The content of NO and the activity of NOS and AchE in brain tissue were measured by colorimetric analysis.The p16 protein expression in brain tissue was measured by ELISA.Results In each-dose group of ASP,the mice＇s learning and memory function was clearly improved,the content of NO and the activity of NOS and AchE,and the expression of p16 were significantly decreased,compared with the D-gal and NaNO2 groups,whose differences were significant.Conclusion ASP has the therapeutic effect of anti-aging,whose mechanism might be related to increasing antioxidant effect and regulating cell cycle.