文献类型：期刊文献

中文题名：当归挥发油对自发性高血压大鼠血管内皮相关信号通路PI3K/Akt/eNOS的影响

英文题名：Effect of Angelica essential oil on PI3K/Akt/eNOS signaling pathway in vascular endothelium of spontaneous hypertensive rats

作者：江华[1];毛玉娟[1];杨锐[1];申进增[1];何亚丽[1];伊琳[1]

第一作者：江华

机构：[1]甘肃中医药大学,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2022

卷号：33

期号：4

起止页码：794

中文期刊名：时珍国医国药

外文期刊名：Lishizhen Medicine and Materia Medica Research

收录：北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：国家自然科学基金(81760803)。

语种：中文

中文关键词：当归挥发油;高血压;血管内皮;磷脂酰肌醇-3-激酶(PI3K);丝氨酸/苏氨酸蛋白激酶(AKT);内皮型一氧化氮合酶(eNOS)

外文关键词：Angelica essential oil;Hypertension;Vascular endothelium;Phosphati-dylinositol 3-kinase(PI3K);Serine/threonine protein kinase(AKT);Endothelial nitric oxide synthase(eNOS)

摘要：目的研究当归挥发油对自发性高血压大鼠血压、胸主动脉血管内皮组织中PI3K、AKT、p-AKT、eNOS、p-eNOS蛋白表达水平的影响,并探讨其相关机制。方法将自发性高血压大鼠随机分为模型组、缬沙坦组、当归挥发油低、中、高剂量组,每组6只;6只同周龄SPF级Wistar大鼠做为正常对照组。缬沙坦组以每日缬沙坦8mg/Kg灌胃,当归低、中、高剂量组每天分别给予当归挥发油0.1ml、0.2ml、0.4ml,与1%、3%、9%的当归挥发油乳剂2.0ml/(kg·d)剂量相当,模型组和正常对照组大鼠分别给予等量蒸馏水灌胃,连续给药4周。采用无创血压检测系统测定给药前及给药后1周、2周、3周、4周各组大鼠的尾动脉收缩压;给药4周后麻醉大鼠,在4℃下分离胸主动脉;通过Western Blot法检测当归挥发油对大鼠胸主动脉血管内皮PI3K、AKT、p-AKT、eNOS、p-eNOS蛋白表达量的变化。结果给药4周后,当归挥发油低剂量组、当归挥发油中剂量组、当归挥发油高剂量组、缬沙坦组大鼠收缩压均低于模型组,差异有统计学意义(P<0.05);Western blot实验结果显示,与模型组比较,当归挥发油低剂量组大鼠AKT、eNOS蛋白表达水平明显上调(P<0.05),PI3K、p-AKT、p-eNOS蛋白表达水平无明显改变(P>0.05);当归挥发油中剂量组大鼠AKT、p-AKT、eNOS、p-eNOS蛋白表达水平明显上调(P<0.05),PI3K蛋白表达水平无明显改变(P>0.05);当归挥发油高剂量组大鼠PI3K、AKT、p-AKT、eNOS、p-eNOS蛋白表达水平明显上调(P<0.05)。结论当归挥发油具有一定的降压作用,其可能是通过调节PI3K/Akt/eNOS信号通路实现降压的。
Objective To study the effects of Angelica sinensis essential oil on blood pressure and PI3 K,AKT,p-AKT,eNOS,and p-eNOS protein expression levels in spontaneous hypertensive rats,and explore their related mechanisms.Methods Spontaneous hypertensive rats were randomly divided into model group,valsartan group,Angelica volatile oil low,medium and high dose groups,6 in each group;6 SPF Wistar rats of the same age as the normal control group.The valsartan group was intragastrically administered with 8 mg/Kg of valsartan daily,and the Angelica low,medium and high dose groups were given 0.1 ml,0.2 ml and 0.4 ml of volatile oil of Angelica per day,and the Angelica containing 1%,3%and 9%The dose of volatile oil emulsion 2.0 ml/(kg·d)was equivalent.Rats in the model group and normal control group were given the same amount of distilled water by gavage for 4 weeks.The non-invasive blood pressure detection system was used to measure the caudal arterial systolic pressure of the rats before and 1 week,2 weeks,3 weeks and 4 weeks after the administration;the rats were anesthetized 4 weeks after the administration and the Thoracic aorta was separated at 4℃;The changes of PI3 K,AKT,p-AKT,eNOS,and p-eNOS protein expressions of volatile oil of Angelica sinensis on thoracic aorta vascular endothelium were detected by Western Blot.Results After 4 weeks of administration,the systolic blood pressure of rats in the low-dose group of Angelica volatile oil,the medium-dose group of Angelica volatile oil,the high-dose group of Angelica volatile oil,and the valsartan group were all lower than that of the model group(P<0.05);The results of Western blot experiments showed that compared with the model group,the expression levels of AKT and eNOS protein in Angelica volatile oil low-dose group were significantly increased(P<0.05),and the expression levels of PI3 K,p-AKT and p-eNOS protein were not significantly changed(P>0.05);The expression levels of AKT,p-AKT,eNOS and p-eNOS protein in the middle-dose group of Angelica volatile oil were significantly increased(P<0.05),and there was no significant change in the expression level of PI3 K protein(P>0.05);PI3 K,AKT,p-AKT,eNOS,p-eNOS protein expression levels were significantly increased in the high-dose group of Angelica volatile oil(P<0.05).Conclusion The volatile oil of Angelica sinensis has a certain antihypertensive effect,which may be achieved by adjusting the PI3 K/Akt/eNOS signaling pathway.