文献类型：期刊文献

中文题名：红芪多糖对糖尿病肾病db/db小鼠肾损伤的改善作用

英文题名：Improving effects of Hedysarum polysaccharide on renal injury in db/db mice with diabetic nephropathy

作者：陈彦旭[1];张磊[1];金智生[1];姜晓雪[1];张钦媛[1];豆鹏程[1];牛媛媛[1];杨娟娟[1]

第一作者：陈彦旭

机构：[1]甘肃中医药大学中医临床学院,甘肃兰州730000

第一机构：甘肃中医药大学中医临床学院

年份：2024

卷号：40

期号：14

起止页码：2078

中文期刊名：中国临床药理学杂志

外文期刊名：The Chinese Journal of Clinical Pharmacology

收录：CSTPCD;;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金地区基金资助项目(82360914);甘肃省高等学校青年博士基金资助项目(2022QB-096);甘肃中医药大学博士研究生创新基金资助项目。

语种：中文

中文关键词：红芪多糖;糖尿病肾病;db/db小鼠;转化生长因子-β1;smad同源物3重组蛋白;smad同源物7重组蛋白

外文关键词：Hedysarum polysaccharides;diabetic nephropathy;db/db mice;transforming growth factor-βi;smad homologue 3 recombinant protein;smad homologue 7 recombinant protein

摘要：目的研究红芪多糖(HPS)对糖尿病肾病(DN)db/db小鼠肾组织中转化生长因子-β1(TGF-β1)、smad同源物3重组蛋白(smad3)、smad7在肾组织表达的影响。方法根据体质量将雄性db/db小鼠随机分为模型组(0.9%NaCl 0.2 mL·d^(-1))、阳性对照组(22.75 mg·kg^(-1)·d^(-1)厄贝沙坦)和高、中、低剂量实验组(200、100、50 mg·kg^(-1)·d^(-1) HPS),每组10只;另取10只同周龄SPF级雄性C57BL/6小鼠作为正常组(0.9%NaCl 0.2 mL·d^(-1))。6组小鼠每天灌胃1次,连续给药12周。于治疗前及治疗后第4、8、12周末检测小鼠血糖水平,用蛋白质印迹法检测TGF-β1、smad3、smad7蛋白的表达水平。结果治疗后,模型组小鼠血糖水平均较正常组显著升高(均P<0.01);高、中剂量实验组小鼠的血糖水平均较模型组显著下降(P<0.05,P<0.01)。正常组、模型组、阳性对照组和高、中剂量实验组的TGF-β1蛋白相对表达水平分别为0.71±0.16、1.66±0.18、1.00±0.17、0.88±0.15和1.23±0.15,smad3蛋白相对表达水平分别为0.89±0.32、2.26±0.35、1.24±0.31、1.05±0.30和1.67±0.35,smad7蛋白相对表达水平分别为1.66±0.03、0.60±0.03、1.10±0.07、1.48±0.08和0.97±0.09。高、中剂量实验组的上述指标与模型组相比,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论HPS可以通过调控血糖水平,抑制TGF-β1、smad3及升高smad7的表达,改善肾纤维化,延缓DN发展进程。
Objective To study the effect of Hedysarum polysaccharides(HPS) on the expression of transforming growth factor-β_1(TGF-β_1), smad homologue 3 recombinant protein(smad3) and smad7 in renal tissue of db/db mice with diabetic nephropathy(DN). Methods According to their body weight, 6-week-old male db/db mice were randomly divided into 5 groups: model group(0.9%NaCl 0.2 mL·d^(-1)), positive control group(22.75 mg·kg^(-1)·d^(-1) irbesartan) and experimental-H,-M,-L groups(200, 100, 50 mg kg^(-1)·d^(-1) HPS), with 10 mice in each group;another 10 SPF grade male C57BL/6 mice of the same week were selected as normal group(0.9%NaCl 0.2 mL·d^(-1)). The mice in the 6 groups were given intragastric administration once a day for 12 weeks. The blood glucose concentration of mice was measured before treatment and at the 4th, 8th and 12th week after treatment. The expression levels of TGF-β1,smad3 and smad7 were detected by Western blotting. Results After treatment,the blood glucose levels of the model group was significantly higher than those of the normal group( all P < 0. 01);compared with the model group,the levels of blood glucose in the experimental-H,-M groups decreased significantly,and the differences were statistically significant( P < 0. 05,P < 0. 01). The relative expression levels of TGF-β1protein in normal group,model group,positive control group and experimental-H,-M groups were 0. 71 ± 0. 16,1. 66 ± 0. 18,1. 00 ± 0. 17,0. 88 ± 0. 15 and 1. 23 ± 0. 15;the relative expression levels of smad3 protein were 0. 89 ± 0. 32,2. 26 ± 0. 35,1. 24 ± 0. 31,1. 05 ± 0. 30 and 1. 67 ± 0. 35;the relative expression levels of smad7 protein were 1. 66 ± 0. 03,0. 60 ± 0. 03,1. 10 ± 0. 07,1. 48 ± 0. 08 and 0. 97 ± 0. 09;there were statistically significant differences between the experimental-H,-M groups and the model group( P < 0. 05,P < 0. 01).Conclusion Hedysarum polysaccharides can improve renal fibrosis and delay the development of diabetic nephropathy by regulating the level of blood glucose,inhibiting TGF-β_1,smad3 and increasing the expression of smad7.