文献类型：期刊文献

中文题名：基于代谢组学的电针对类风湿关节炎寒证模型家兔尿液内源性代谢物的影响

英文题名：Effect of Electroacupuncture on Endogenous Metabolites in Urine of Rabbits with Cold Syndrome of Rheumatoid Arthritis Based on Metabonomics

作者：陈平[1];杜小正[2];王海东[1];田杰祥[3];李向军[1];袁博[3]

第一作者：陈平

机构：[1]甘肃省中医院,甘肃兰州730050;[2]甘肃中医药大学,甘肃兰州730000;[3]甘肃中医药大学附属医院,甘肃兰州730000

第一机构：甘肃省中医院,甘肃兰州730050

年份：2021

卷号：28

期号：6

起止页码：70

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2021_2022】；

基金：国家自然科学基金(81960832);甘肃省高等学校科研项目(2016B-061);兰州市科学技术局(2016-RC-50)。

语种：中文

中文关键词：电针;类风湿关节炎;代谢组学;气相色谱-四级杆飞行时间质谱;兔

外文关键词：electroacupuncture;rheumatoid arthritis;metabonomics;gas chromatography-quadrupole time of flight mass spectrometry;rabbits

摘要：目的通过分析类风湿关节炎寒证模型家兔尿液内源性代谢物,基于代谢组学探讨电针治疗类风湿关节炎的作用机制。方法将24只家兔随机分为正常组、模型组和电针组,每组8只,卵蛋白诱导联合低温冷冻制备类风湿关节炎寒证家兔模型。电针"足三里",留针30 min,1次/d,共7 d。干预结束后收集新鲜尿液,气相色谱-四级杆飞行时间质谱采集家兔尿液代谢物图谱,结合多元统计方法分析数据;记录家兔膝关节周径,测定各组痛阈值,观察膝关节滑膜组织病理形态。结果与正常组比较,模型组家兔膝关节周径明显增大,痛阈值明显降低(P<0.05);与模型组比较,电针组家兔膝关节周径明显减小,痛阈值明显升高(P<0.05)。模型组较正常组丙酮酸、乳酸、柠檬酸、花生四烯酸、牛磺酸、马尿酸、色氨酸及尿酸水平相对升高,甘氨酸、苯丙氨酸水平相对降低;电针组较模型组甘氨酸、苯丙氨酸水平上调,其余代谢物水平均下调。模型组较正常组滑膜组织滑膜衬里层细胞明显增生,层数增多,滑膜组织呈绒毛状,滑膜内可见微血管扩张、充血、水肿及炎性细胞浸润;电针组滑膜细胞增生层数较少,炎性细胞浸润程度及滑膜纤维组织增生程度较模型组明显减轻。结论电针可缓解膝关节肿胀,提高痛阈,改善滑膜组织病理形态,调节类风湿关节炎家兔机体能量代谢、脂肪酸代谢、氨基酸代谢及嘌呤代谢。
Objective To explore the metabolomics mechanism of electroacupuncture treatment for rheumatoid arthritis by analyzing the endogenous metabolites in urine of rabbits of rheumatoid arthritis with cold syndrome. Methods A total of 24 rabbits were randomly divided into normal group, model group and electroacupuncture group, 8 rabbits in each group. Rheumatoid arthritis with cold syndrome rabbit model was made with egg protein induction and low temperature freezing method. Electroacupuncture group was applied to "Zusanli" acupoint once a day for 30 minutes for 7 days. The fresh urine was collected and the urine metabolite profiles were collected by gas chromatography-quadrupole time-of-flight mass spectrometry, and the data were statistically analyzed by multivariate statistical analysis method after the intervention;the knee joint circumference of rabbits was recorded;the pain threshold of each group was measured;the pathological changes of knee joint synovial tissue were observed. Results Compared with the normal group, the rabbit knee joint circumference in the model group significantly increased, and the pain threshold was significantly reduced(P<0.05). Compared with the model group, the circumference of the knee joint of the rabbits in the electroacupuncture group was significantly reduced, and the pain threshold significantly increased(P<0.05). Compared with the normal group, the levels of pyruvate, lactic acid, citric acid, arachidonic acid, taurine, hippuric acid, tryptophan and uric acid in the model group were relatively higher, while the levels of glycine and phenylalanine were relatively lower. Compared with the model group, the levels of glycine and phenylalanine increased, while the other metabolite levels decreased in the electroacupuncture group. Compared with the normal group, the synovial lining cells in the synovial tissue of the model group were significantly proliferated, the number of layers increased, the synovial tissue was villi-like, and microvascular expansion, hyperemia, edema and inflammatory cell infiltration were seen in the synovium;the electroacupuncture group had fewer synovial cell proliferation layers, and the degree of inflammatory cell infiltration and synovial fibrous tissue proliferation were significantly reduced compared with the model group. Conclusion Electroacupuncture can relieve knee joint swelling, increase pain threshold, improve pathological changes of synovial tissue, regulate energy metabolism, fatty acid metabolism, amino acid metabolism and purine metabolism in rabbits of rheumatoid arthritis.