文献类型：期刊文献

中文题名：菖蒲郁金汤对抽动障碍模型大鼠纹状体铁稳态的影响

英文题名：Effects of Changpu Yujin Decoction(菖蒲郁金汤)on iron homeostasis in the striatum of rats with a tic disorder model

作者：孙铭阳[1];李玉霞[2];黄爽[1];黄立威[1];李梦雪[1];崔敏[1]

第一作者：孙铭阳

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2026

卷号：37

期号：7

起止页码：1262

中文期刊名：时珍国医国药

外文期刊名：JOURNAL OF LI-SHIZHEN TRADITIONAL CHINESE MEDICINE

收录：;北大核心:【北大核心2023】；

基金：第五批全国中医临床优秀人才研修项目(国中医人教涵[2022]1号);甘肃省自然科学基金(23JRRA1581)。

语种：中文

中文关键词：抽动障碍;多巴胺;铁稳态;菖蒲郁金汤

外文关键词：Tic disorder;Dopamine;Iron homeostasis;Changpu Yujin Decoction(菖蒲郁金汤)

摘要：目的 观察菖蒲郁金汤对抽动模型大鼠纹状体铁稳态相关因子的影响。方法 将60只雄性SD大鼠随机分至空白组(n=15)和造模组(n=45),采用亚氨基二丙腈(IDPN)腹腔注射法建立动物模型,进行行为学评价,评分≥2分,即抽动障碍大鼠造模成功。造模成功后,将符合标准的45只SD大鼠按完全随机分为模型组、硫必利组及菖蒲郁金汤组,连续干预4周。在造模后、干预后对大鼠进行行为学观察;尼氏染色法观察大鼠纹状体组织形态结构;透射电镜观察大鼠纹状体神经元超微结构;铁检测试剂盒检测大鼠纹状体及外周血铁含量;Elisa法检测大鼠纹状体多巴胺(DA)、酪氨酸羟化酶(TH)、活性氧(ROS)含量;免疫组化法、RT-PCR法、WB法检测大鼠纹状体组织中转铁蛋白受体1(TFR1)、二价金属离子转运蛋白(DMT1)、膜铁转运蛋白1(FPN1)、铁调素(hepcidin)、线粒体铁蛋白(FtMt)、铁蛋白(FT)mRNA及蛋白表达。结果 与空白组比较,模型组大鼠刻板、运动行为显著升高(P<0.01);DA、TH含量降低(P<0.01),ROS含量升高(P<0.01);TFR1、DMT1、Hepcidin蛋白及mRNA表达升高(P<0.01),FPN1、FtMt、FT蛋白及mRNA表达降低(P<0.01);纹状体铁含量及血清铁含量降低(P<0.01);神经细胞形态结构异常;神经元超微结构破坏。与模型组比较,硫必利组与菖蒲郁金汤组大鼠刻板、运动行为显著降低(P<0.01);DA、TH含量升高(P<0.01),ROS含量降低(P<0.01);TFR1、DMT1、Hepcidin蛋白及mRNA表达降低,FPN1、FtMt、FT蛋白及mRNA表达升高;纹状体铁含量及血清铁含量升高(P<0.01);神经细胞形态结构较规则;神经元超微结构完整,且菖蒲郁金汤组优于硫必利组。结论 菖蒲郁金汤具有抗抽动的作用,其机制可能与通过调控纹状体铁稳态进而调节DA系统有关。
Objective To observe the effects of Changpu Yujin Decoction(菖蒲郁金汤,CPYJD)on the factors related to striatal iron homeostasis in tic disorder(TD)model rats.Methods Sixty male SD rats were randomly divided into the blank group(n=15)and the modeling group(n=45),and the animal model was established by intraperitoneal injection of 3,3-iminodipropionitrile(IDPN),and behavioral evaluation was performed,and a score of≥2 meant that the modeling of the TD rats was successful.After successful model?ing,45 SD rats meeting the criteria were divided into the model group,the Tiapride group and the CPYJD group by complete randomization,and the intervention was carried out for 4 consecutive weeks.Behavioral observations were performed on rats after modeling and post-intervention;Nissl staining was used to observe the morphology and structure of rat striatal tissue;transmission electron microscopy(TEM)was used to observe the ultrastructure of rat striatal neurons;an iron assay kit was used to detect the iron content in rat striatum and peripheral blood;ELISA was used to detect the content of dopamine(DA),tyrosine hydroxylase(TH),and reactive oxygen species(ROS)in rat striatum;Immunohistochemistry(IHC),RT-PCR and Western blot(WB)were used to detect the mRNA and protein expression of transferrin receptor 1(TFR1),divalent metal transporter 1(DMT1),ferroportin 1(FPN1),hepcidin,mitochondrial ferritin(FtMt),ferritin(FT)in the striatum of rats.Results Compared with the blank group,rats in the model group showed significantly higher stereotyped and locomotor behaviors(P<0.01);lower DA and TH levels and higher ROS levels(all P<0.01);higher expression of TFR1,DMT1 and Hepcidin protein levels and mRNA levels(P<0.01),and lower expression of FPN1,FtMt and FT protein levels and mRNA levels(P<0.01);decreased striatal and serum iron levels(P<0.01);abnormal morphology and structure of nerve cells;and destruction of neuronal ultrastructure.Compared with the model group,the stereotyped and locomotor behaviors of rats in the Tiapride group and the CPYJDgroup were significantly reduced(P<0.01);the DA and TH levels were elevated(P<0.01),and the ROS level was reduced(P<0.01);the protein and mRNA levels of TFR1,DMT1,and Hepcidin were reduced,while the protein and mRNA levels of FPN1,FtMt,and FT were increased;striatal iron content and serum iron content increased(P<0.01);neuronal cell morphology and structure were more regular;neuronal ultrastructure was intact,and CPYJD group was better than the Tiapride group.Conclusion CPYJD has an anti-twitching effect,and the mechanism may be related to regulating the DA system by modulating striatal iron homeostasis.