文献类型：期刊文献

中文题名：不同花色铁棒锤基因组DNA提取及遗传差异分析

英文题名：Genomic DNA Extraction and Diversity Analysis for Types of Aconitum pendulum Busch

作者：朱田田[1,2];杜弢[1,2];陈红刚[1,2];晋玲[1,2];林丽[1,2];侯嘉[1,2]

第一作者：朱田田

机构：[1]甘肃中医学院药学系,甘肃兰州730000;[2]甘肃省高校中(藏)药化学质量研究省级重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2012

卷号：40

期号：4

起止页码：316

中文期刊名：山西农业科学

外文期刊名：Journal of Shanxi Agricultural Sciences

收录：CSTPCD

基金：甘肃省教育厅科研项目(0906B-07);甘肃中医学院中青年科研基金项目(09ZQ-17)

语种：中文

中文关键词：铁棒锤;DNA;CTAB;RAPD-PCR;遗传学差异

外文关键词：Aconitum pendulum Bush; DNA; CTAB; RAPD-PCR; genetic diversity

摘要：利用RAPD-PCR技术对不同花色的铁棒锤植物进行遗传差异分析,旨在为今后铁棒锤的引种驯化及品种选育提供参考。采用CTAB法,从铁棒锤植物不同材料提取基因组DNA,用琼脂糖凝胶电泳法和紫外分光光度法检测所得总DNA的浓度和纯度,并用20条RAPD随机引物进行PCR扩增及琼脂糖凝胶电泳分析。结果表明,CTAB法从铁棒锤的新鲜叶片及干燥种子中均可提取出基因组DNA,但前者DNA的质量好、产量高;有3条引物可以扩增出条带,其中,引物E68629的扩增产物清晰稳定,可用于铁棒锤遗传差异分析。RAPD分析结果表明,2种花色铁棒锤在分子水平上具有遗传学差异。
Genetic diversity for types of A conitum pendulum Busch was analyzed with RAPD-PCR technology and CTAB method was used to extract the genomic DNA from Aconitump pendulum Busch. The yield and purity of total genomic DNA were examined by agarose gel eleetrophoresis and UV-spectrephotometry;PCR amplification and agarose gel electrophoresis analysis with 20 RAPD random primers. The results were the following： CTAB method could extract the genomic DNA from fresh leaf and dry seeds of Aconitum pendulum Busch, but the former had good quality and high output;Three primers amplified bands could get primer, in which the product of primer E68629 was clear and stabe, and could be used in genetic diversity analysis of Aconitum pendulum Busch. The results showod that two types of Aconitum pendulum Busch had genetic diversity at molecular level.