详细信息
平胃胶囊对肝郁脾虚型反流性食管炎大鼠食管黏膜炎症的影响
Effects of Pingwei capsules on esophageal mucosal inflammation in Ganyu Pixu reflux esophagitis rats
文献类型:期刊文献
中文题名:平胃胶囊对肝郁脾虚型反流性食管炎大鼠食管黏膜炎症的影响
英文题名:Effects of Pingwei capsules on esophageal mucosal inflammation in Ganyu Pixu reflux esophagitis rats
作者:温东升[1];张晶[1,2];毛兰芳[2];刘俊宏[2];汪龙德[2]
第一作者:温东升
机构:[1]甘肃中医药大学,甘肃兰州730020;[2]甘肃中医药大学附属医院,甘肃兰州730020
第一机构:甘肃中医药大学
年份:2024
卷号:39
期号:4
起止页码:377
中文期刊名:华西药学杂志
外文期刊名:West China Journal of Pharmaceutical Sciences
收录:CSTPCD;;北大核心:【北大核心2023】;CSCD:【CSCD_E2023_2024】;
基金:甘肃省自然科学基金项目(21JR1RA269)。
语种:中文
中文关键词:反流性食管炎;核因子κB抑制蛋白激酶β/核因子κB抑制蛋白α/核因子κB信号通路;肝郁脾虚;枳术宽中胶囊;莫沙必利;平胃胶囊;食管黏膜炎症;炎症因子
外文关键词:Reflux esophagitis;IKκβ/lkBo/NF-κB signaling pathway;Ganyu Pixu;Zhizhu Kuanzhong capsules;Mosapride;Pingwei capsules;Esophageal mucosal inflammation;Inflammatory factors
摘要:目的研究平胃胶囊对反流性食管炎模型大鼠核因子κB(NF-κB)/p65相关因子表达的影响。方法采用食管十二指肠侧侧吻合术+饥饱失常+夹尾刺激制备大鼠肝郁脾虚型反流性食管炎模型。将大鼠分为正常组、模型组、平胃胶囊组(低、中、高剂量)、枳术宽中胶囊组、莫沙必利组,每日2次,共4周。镜下及肉眼观察评价食管的组织病变,ELISA法、WesternBlot、Rt-qPCR法检测相关因子的表达水平。结果平胃胶囊可显著改善模型大鼠食管的黏膜炎症,降低血清中炎性因子白细胞介素-1β(IL-1β)、肿瘤坏死因子-α、IL-6及食管组织中NF-κB抑制蛋白激酶β(IKKβ)、p65、环氧化酶2蛋白的水平,升高NF-κB抑制蛋白α(IκBα)表达。结论平胃胶囊通过抑制胃组织中IKKβ/IκBα/NF-κB通路,降低血清中炎症因子的水平,减轻慢性胃炎大鼠的胃黏膜损伤。
OBJECTIVE To study the effect of Pingwei capsule on the expression of nuclear factorκB(NF-κB)/p65-related factors in reflux esophagitis model rats.METHODS Lateral esophagoduodenal anastomosis+hunger and satiety disorders+pinch-tail stimulation were used for the preparation of Ganyu Pixu reflux esophagitis model.It was divided into normal group,model group,low,medium and high dose group of Pingwei capsules,Zhizhu Kuanzhong capsules group,and mosapride group,twice a day for 4 weeks.Microscopic and naked eye observation was used to evaluate the esophageal tissue lesions,and the expression levels of related factors were detected by ELISA,Western Blot and Rt-qPCR.RESULTSSPingwei capsules could significantly improve the inflammation of esophageal mucosa in RE rats,reduce serum inflammatory factors such as IL-1β,TNF-αand IL-6,and downregulate esophageal tissue IKKβ,p65,COX2 protein levels,elevated IkBαexpression.CONCLUSION Pingwei capsules reduce gastric mucosal injury in rats with chronic gastritis by inhibiting IKKβ/IκBo/NF-κB pathway in gastric tissues and lowering serum inflammatory factor levels.
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