文献类型：期刊文献

英文题名：Flowering characteristics and mating system of Fritillaria cirrhosa (Liliaceae), an endangered plant in China

作者：Ma, Yanzhu[1];Cui, Zhijia[1,2,3];Cheng, Chun Ya[1];Li, Ran[1];Wu, Haixu[1];Jin, Ling[1,2,3];Ma, Yi[1,2,3];Wang, Zhenheng[1,2,3]

第一作者：Ma, Yanzhu

通信作者：Cui, ZJ[1];Jin, L[1];Cui, ZJ[2];Jin, L[2];Cui, ZJ[3];Jin, L[3]

机构：[1]Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]Northwest Collaborat Innovat Ctr Tradit Chinese M, Lanzhou 730000, Gansu, Peoples R China;[3]MOE PRC, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Coll Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]corresponding author), Northwest Collaborat Innovat Ctr Tradit Chinese M, Lanzhou 730000, Gansu, Peoples R China;[3]corresponding author), MOE PRC, Lanzhou 730000, Gansu, Peoples R China.|[1073501e14fb35863569f]甘肃中医药大学药学院（西北中藏药协同创新中心办公室）;[10735]甘肃中医药大学;

年份：2022

卷号：45

期号：4

起止页码：1307

外文期刊名：BRAZILIAN JOURNAL OF BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000871169100001)】；

基金：This research was supported by the financial support of the 'Double First-Class' Research Key Project of Gansu (GSSYLXM-05), Ecological Planting and Quality Assurance Project of Genuine Medicinal Materials (2020 No. 153) and Gansu Province Higher Education Innovation Fund Project (2021A-083).

语种：英文

外文关键词：Flowering dynamics; Fritillaria cirrhosa; Mating system; Phenology; Traits

摘要：Fritillaria cirrhosa D. Don is an endangered species of the family Liliaceae that has economic, medicinal, and ecological values, as well as the source of the traditional Chinese medicine 'Fritillariae Cirrhosae Bulbus.' Fritillariae Cirrhosae Bulbus is one of the traditional precious Chinese medicines in China, which has the medicinal value of clearing away heat and moistening the lungs, resolving phlegm and relieving cough, dispelling knots, and eliminating carbuncle. In this study, the phenology, flowering dynamics, and floral traits of F. cirrhosa were observed in the field, and the related morphological indicators were measured. The pollen viability and stigma receptivity during flowering of F. cirrhosa were measured by MTT method and benzidine-hydrogen peroxide method, respectively, and the microstructures of pollen grains were observed using scanning electron microscopy. At the same time, the mating system of F. cirrhosa was evaluated by measuring the pollen and ovule ratio (P:O), determining the outcrossing index (OCI), and performing bagging experiments. Based on these observations, we found that the phenology of F. cirrhosa was from late April to mid-August, the average duration of 117 days, divided into eight stages from germination to fructescence, of which the population flowering lasted 31 days. The flowering period of F. cirrhosa is divided into initial budding stage, middle budding stage, late budding stage, initial bloom stage, full bloom stage, and final bloom stage. Most of the anthers of F. cirrhosa were longitudinally split and released pollens from the proximal end of the anthers to the top from the second day of flowering, and the anthers corresponding to the outer corolla began to release pollens first. As the anthers dehisced and pollens were released, and the stamens were gradually lower than the pistils. The pollen grains of F. cirrhosa were prolate, which had the reticulated surface and various mesh sizes. The pollen viability and stigma receptivity of F. cirrhosa increased at first and then decreased with the flowering stage. Combined with P:O = 2891.6 +/- 373.4, OCI = 4 and the results of the bagging experiments, we determined that F. cirrhosa was generally outcrossing. These results provide the basis for the cultivation, breeding, and conservation of this rare and charismatic plant, helping to reveal the nature of its sexual reproduction process, explore the possible endangerment reasons from the perspective of reproductive biology, and provide a scientific basis for its protection and utilization.