文献类型：期刊文献

中文题名：半夏试管块茎延缓生长保存技术

英文题名：Conservation Technology of In Vitro Tubers of Pinellia ternata by Delayed Growth

作者：张延红[1,2];张亚萍[1];王琳佳[1];何春雨[1,2,3];郭清毅[1,2,3]

第一作者：张延红

机构：[1]甘肃中医药大学药学院,兰州730000;[2]天然药用种质资源挖掘与新品种选育协同创新中心,兰州730000;[3]甘肃中医药大学杏林百草园,兰州730000

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2026

卷号：46

期号：1

起止页码：101

中文期刊名：植物研究

外文期刊名：Bulletin of Botanical Research

收录：;北大核心:【北大核心2023】；

基金：甘肃省高等学校产业支撑计划项目(2020C-09);甘肃省联合基金项目(24JRRA877);甘肃省重点研发计划项目(24YFNA007);甘肃中医药大学成果转化培育项目(2023CGZH-18);甘肃中医药大学教学改革项目(ZHXM-2021-17)。

语种：中文

中文关键词：半夏;试管块茎;延缓生长;再生植株;淀粉粒分布;遗传稳定性

外文关键词：Pinellia ternata;in vitro tuber;delayed growth preservation;regenerated plants;starch granule distribution;genetic stability

摘要：为建立半夏(Pinellia ternata)试管块茎延缓生长保存技术体系,该研究采用不同蔗糖质量浓度、蔗糖和甘露醇配比、低温方法保存半夏试管块茎,360 d后恢复生长,观察统计其生长指标,并采用石蜡组织切片技术研究块茎中淀粉粒的变化,采用ISSR-PCR和RAPD分子标记技术检测保存后再生植株的遗传稳定性。结果表明:成熟块茎接种在新鲜的1/2MS+30 g·L^(-1)蔗糖+60 g·L^(-1)甘露醇的培养基上4℃保存360 d,恢复生长后块茎的成苗率高达96.67%,植株生长良好,保存效果最好;90 g·L^(-1)蔗糖处理的块茎恢复生长后成苗率高达90.00%,但因渗透压过高生长缓慢。成熟块茎在原培养基1/2MS+30 g·L^(-1)蔗糖中4℃保存360 d,恢复生长后块茎的成苗率最低,仅为36.70%。组织学研究表明,保存后死亡的块茎,叶原基少,细胞中几乎无淀粉粒,无黏液细胞。成苗率高和成苗率低的处理恢复生长的块茎中均储藏有大量的淀粉粒,但成活率高的处理茎尖叶原基层数更多,黏液细胞数量较少,植株生长更旺盛。采用分子标记技术共扩增条带1140条,均未检测到变异条带,表明延缓生长保存后再生植株遗传稳定。该研究建立了简便、高效的半夏延缓生长保存技术,为半夏种质资源中短期保存提供了一条可行途径。
To establish a technology system for the delayed growth preservation of Pinellia ternata tubers in vitro,this study valuated different sucrose mass concentrations,the ratio of sucrose to mannitol,and lowtemperature methods to preserve the in vitro tubers.After 360 days,the tubers were allowed to resume growth,and the growth indicators were observed and statistically analyzed.Starch grain dynamics in the tubers were examined using paraffin sectioning technique.The genetic stability of regenerated plants after preservation was assessed using ISSR-PCR and RAPD molecular markers.Mature tubers were inoculated on fresh 1/2MS+30 g?L^(-1)sucrose+60 g?L^(-1)mannitol medium and stored at 4℃for 360 days,the sprouting rate of tubers after recovery growth was as high as 96.67%,and the plants grew well with the best preservation effect.Tubers cultured on medium containing 90 g?L^(-1)sucrose,showed reduced growth despite a sprouting rate of 90.00%,likely due to the excessively high osmotic pressure.When mature tubers were stored in the old medium of 1/2MS+30 g?L^(-1)sucrose at 4℃for 360 days,the sprouting rate of tubers after recovery growth was the lowest,only 36.70%.no starch grains in the cells,and no mucilage cells.Both the high sprouting rate and low sprouting rate treatments had a large amount of starch grains stored in the tubers after recovery growth,but the treatment with high survival rate had more layers of leaf primordia in the stem tips and fewer mucilage cells,and the plants grew more vigorously.A total of 1140 bands were amplified using molecular marker techniques,and no variant bands were detected,indicating genetic stability of regenerated plants after delayed growth preservation.This study established a simple and efficient technique for the delayed growth preservation of P.ternata in vitro tubers,providing a feasible approach for short-term preservation of P.ternata germplasm.