文献类型：期刊文献

英文题名：To study the protective effect of Huangqi Baihe Granules on Radiation brain injury based on network pharmacology and experiment

作者：Liu, Xiuzhu[1];Li, Jiawei[1];Wang, Yanru[1];Wu, Bingbing[2];Wang, Siyu[1];Guo, Qingyang[1];Liu, Yongqi[1,3]

第一作者：Liu, Xiuzhu

通信作者：Liu, YQ[1]

机构：[1]Gansu Univ Chinese Med, Gansu Univ, Key Lab Mol Med & Chinese Med Prevent & Treatment, Lanzhou 730000, Gansu, Peoples R China;[2]940th Hosp Chinese Peoples Liberat Army Joint Supp, Lanzhou 730050, Gansu, Peoples R China;[3]Gansu Univ Tradit Chinese Med, Sch Basic Med Sci, Lanzhou 730050, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Univ Tradit Chinese Med, Sch Basic Med Sci, Lanzhou 730050, Peoples R China.|[107351d2d02a88e1f325f]甘肃中医药大学基础医学院（敦煌医学研究所）;[10735]甘肃中医药大学;

年份：2023

卷号：315

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-85160718995);WOS:【SCI-EXPANDED(收录号:WOS:001011587700001)】；

语种：英文

外文关键词：Radiation brain injury; Network pharmacology; Huangqi baihe Granules; Oxidative stress; Inflammation

摘要：Ethnopharmacological relevance: Huangqi baihe Granules (HQBHG), which is a key Chinese medical prescription, has a remarkable efficacy in oxidative stress and inflammation. Nevertheless, the therapeutic effect on Radiation brain injury (RBI) has rarely been studied.Aim of the study: The study aimed to verify the effect of HQBHG against RBI and explore its potential mechanism. Methods: The potential targets and mechanisms of HQBHG against RBI were predicted by network pharmacology and verified by established rat model of RBI Firstly, the therapeutic effect of HQBHG in RBI was confirmed by water maze test, HE staining and Enzyme-linked immunosorbent assay (ELISA). Secondly, the potential critical anti-RBI pathway of HQBHG was further explored by water maze, HE staining, immunofluorescence assays, ELISA and western blot. Results: A total of 43 HQBHG anti-RBI targets were obtained. Gene Ontology (Go) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotations showed that the treatment of HQBHG in RBI might be mainly related to oxidative stress, inflammation and PI3K/AKT pathway. Experimental studies have indicated that HQBHG can improve spatial learning and memory ability, alleviate pathological damage of brain tissue in RBI of rats. HQBHG also can down-regulate the levels of IL-1 & beta;, TNF-& alpha;, ROS and MDA, meanwhile, GSH was significantly up-regulated. In addition, the HQBHG can increase the protein expression phosphorylations PI3K (p-PI3K), phosphorylations AKT(p-AKT) and Nrf2 in the brain tissue of RBI. Conclusion: HQBHG may alleviated RBI by regulated oxidative stress and inflammatory response through PI3K/ AKT/Nrf2 pathway.