文献类型：期刊文献

中文题名：益气健脾中药对脾气虚大鼠神经肽Y、血管活性肠肽和丝裂原活化蛋白激酶14基因表达的影响

英文题名：Effect of Yiqi Jianpi Herb on Content of NPY, VIP and Expression of Mapk14 mRNA of Rats with Spleen-qi Deficiency

作者：段永强[1,2];程卫东[2];杜娟[1];杨晓轶[1];刘靓[1];巩子汉[1];朱立鸣[1];成映霞[1];李兰珍[1]

第一作者：段永强

机构：[1]甘肃中医学院甘肃省中药药理与毒理学重点实验室;[2]兰州大学基础医学院中西医结合研究所

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2014

卷号：21

期号：4

起止页码：59

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2013_2014】；

基金：国家自然科学基金(81160420);甘肃省自然科学基金(1010RJZA148);甘肃省教育厅基金(1006-05)

语种：中文

中文关键词：脾气虚证;血管活性肠肽;丝裂原活化蛋白激酶;四君子汤;大鼠

外文关键词：神经肽Y;spleen-qi deficiency;NPY;vasoactive intestinal peptide;Mapk;Sijunzi decoction;rats

摘要：目的观察脾气虚大鼠血清、小肠神经肽Y(NPY)和血管活性肠肽(VIP)含量,小肠组织丝裂原活化蛋白激酶14(Mapk14)mRNA表达的变化及益气健脾中药的干预效应,以揭示脾气虚证发生的内在机制。方法受试动物随机分为空白组、模型组(7、14、21 d组)、益气健脾组,每组10只。除空白组外,其余各组采用大黄法、力竭法及饥饿法复合法建立脾气虚证大鼠模型,造模同时益气健脾组每日给予四君子汤20 g/kg干预21 d,各组大鼠分时段采用放免法测定小肠、血清NPY和VIP含量,采用实时荧光定量PCR检测小肠组织Mapk14 mRNA表达水平。结果与空白组比较,模型组大鼠血清及小肠组织NPY含量降低(P<0.05),VIP含量升高(P<0.05),小肠组织Mapk14 mRNA相对表达量升高(P<0.05),且以模型21 d组显著;与模型21 d组比较,益气健脾组大鼠血清及小肠组织NPY含量升高(P<0.05),VIP含量降低(P<0.05),小肠组织Mapk14 mRNA相对表达量降低(P<0.05)。结论益气健脾中药具有调节脑肠肽NPY、VIP分泌及抑制小肠组织Mapk14 mRNA异常表达的作用。
Objective To investigate the effect of Yiqi Jianpi herb on content of NPY, VIP and expression of Mapk14 mRNA of rats with spleen-qi deficiency. Methods Rats were randomly divided into normal groups, model group （observed on 7 d, 14 d and 21 d）, treatment group of Yiqi Jianpi herb, 10 rats in each group. The spleen-qi deficient model rats were made by rhubarb, exhaustive and hungry method, and treatment group was treated with Sijunzi decoction （20 g/kg） for 21 d, then the content of NPY, VIP in serum and small intestine were evaluated with radioimmunoassay, and expression of Mapk14 mRNA in small intestine tissue was evaluated with real time fluorescent quantitative PCR. Results In model group, content of NPY was much lower than that of normal group （P〈0.05）, content of VIP and relative expression of Mapk14 mRNA in small intestine tissue were much higher than that of normal group （P〈0.05）, the difference was obvious in 21 d group. Compared with model 21 d group, content of NPY was increased （P〈0.05）, content of VIP and relative expression of Mapk14 mRNA in small intestine tissue were decreased （P〈0.05） in treatment group. Conclusion Yiqi Jianpi herb has functions of adjusting NPY, VIP secretion and inhibiting abnormal expression of Mapk14 mRNA.