文献类型：期刊文献

中文题名：麻杏石甘汤含药血清对阿霉素诱导H9C2心肌细胞损伤的保护作用

英文题名：Protective effect of serum containing Maxing Shigan Decoction(麻杏石甘汤)on doxorubicin-induced injury in H9C2 cardiomyocytes

作者：刘小瑞[1,2];王斯琦[1,2];苏敬[2];杨丽丽[1,2];任珂[1,2];刘东玲[1,2]

第一作者：刘小瑞

机构：[1]甘肃中医药大学陇药产业创新研究院,甘肃兰州730000;[2]甘肃中医药大学药学院,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2025

卷号：36

期号：17

起止页码：3247

中文期刊名：时珍国医国药

外文期刊名：JOURNAL OF LI-SHIZHEN TRADITIONAL CHINESE MEDICINE

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金面上项目(82074419);甘肃省杰出青年基金(23JRRA1203)。

语种：中文

中文关键词：麻杏石甘汤含药血清;阿霉素;心肌损伤;细胞凋亡

外文关键词：Maxing Shigan Decoction(麻杏石甘汤)-contained serum;Doxorubicin;Myocardial injury;Apoptosis

摘要：目的探究麻杏石甘汤对阿霉素诱导H9C2心肌细胞损伤的保护作用。方法通过网络药理学与分子对接的方法预测麻杏石甘汤防治阿霉素心肌损伤的潜在作用机制;建立阿霉素诱导细胞损伤模型并用麻杏石甘汤含药血清干预H9C2细胞,采用MTT、Hoechst染色法检测细胞的存活率、细胞凋亡及形态变化,采用试剂盒检测细胞中LDH、MDA、SOD的活性,Western blot检测Bcl-2、Bax、Caspase-3等蛋白表达。结果网络药理学结果表明麻杏石甘汤主要参与活性氧、IL-17信号通路、凋亡、肿瘤坏死因子信号通路、细胞周期相关信号通路。分子对接结果显示,与CASP3、CASP8、BCL2对接最好的成分分别是芒柄花素、甘草次酸、槲皮素。麻杏石甘汤含药血清进行干预能够增加阿霉素诱导的细胞存活率(P<0.01),降低细胞凋亡率(P<0.01),LDH、MDA含量(P<0.01),增加SOD含量(P<0.01),Caspase-3、Bax蛋白表达明显降低(P<0.01),Bcl-2蛋白表达明显增加(P<0.01)。结论麻杏石甘汤含药血清对阿霉素诱导的心肌细胞具有保护作用,其机制可能是通过抗氧化与抑制心肌细胞的凋亡而保护心肌细胞。
Objective To investigate the protective effect of Maxing Shigan Decoction(麻杏石甘汤,MXSGD)on Doxorubicin(DOX)-induced H9C2 cardiomyocyte injury.Methods The potential mechanism of MXSCD in preventing and treating DOX-induced myocardial injury was predicted using network pharmacology and molecular docking.A DOX-induced H9C2 cell injury model was established and intervened with MXSCD-contained serum.Cell viability,apoptosis,and morphological changes were detected using MTT and Hoechst staining assays.The activities of LDH and SOD,and the level of MDA in cells were measured using the assay kits.The protein expression levels of Bcl-2,Bax,and Caspase-3 were detected by Western blot(WB).Results Network pharmacology results indicated that MXSGD primarily involves reactive oxygen species(ROS),IL-17 signaling pathway,apoptosis,tumor necrosis factor signaling pathway,and cell cycle-related signaling pathways.Molecular docking results showed that the components with the best docking scores to CASP3,CASP8,and BCL2 were formononetin,glycyrrhetinic acid,and quercetin,respectively.Intervention with MXSCD-contained serum significantly increased the viability of DOX-induced cells(P<0.01),reduced the apoptosis rate(P<0.01),decreased LDH activity and MDA levels(P<0.01),increased SOD activity(P<0.01),downregulated Caspase-3 and Bax protein expression(P<0.01),and upregulated Bcl-2 protein expression(P<0.01).Conclusion MXSCD-contained serum had the protective effect on DOX-induced cardiomyocytes,possibly through exerting antioxidant effects and inhibiting cardiomyocyte apoptosis.