文献类型：期刊文献

中文题名：甘西鼠尾草注射液与丹参注射液对PC12细胞H_2O_2损伤保护作用比较研究

英文题名：Comparison of protective effects on H_2O_2-induced PC12 injury from S.miltiorrhiza Bunge injection and S.przewalskii Maxim injection

作者：罗慧英[1];程体娟[2]

第一作者：罗慧英

机构：[1]甘肃中医学院药理教研室;[2]兰州大学药学院药理教研室

第一机构：甘肃中医药大学药学院（西北中藏药协同创新中心办公室）

年份：2009

卷号：14

期号：9

起止页码：1036

中文期刊名：中国临床药理学与治疗学

外文期刊名：Chinese Journal of Clinical Pharmacology and Therapeutics

收录：CSTPCD;;CSCD:【CSCD2011_2012】；

基金：甘肃省教育厅资助科研项目(No:013-01)

语种：中文

中文关键词：甘西鼠尾草注射液;丹参注射液;PC12;H2O2;bcl-2;bax

外文关键词：S.miltiorrhiza Bunge injection（SMBI）; S.przewalskii Maxim injection（SPMI）; H2O2; apoptosis; bcl-2; bax

摘要：目的:比较甘西鼠尾草注射液与丹参注射液对PC12细胞H2O2损伤的保护作用。方法:采用细胞株培养的方法,建立PC12细胞H2O2损伤模型,通过MTT法和流式细胞分析仪检测细胞存活率和凋亡率;采用免疫细胞化学染色法检测PC12细胞bcl-2和bax表达。结果:PC12细胞经H2O2损伤后,细胞存活率降低、凋亡率增加,甘西鼠尾草注射液与丹参注射液处理组均可有效缓解上述改变(P<0.05);两药均使bcl-2表达增加(P<0.05),bax表达减少(P<0.05)。结论:甘西鼠尾草注射液与丹参注射液对PC12细胞H2O2损伤具有明显保护作用。其机理可能与增强凋亡抑制因子bcl-2表达、抑制凋亡促进因子bax表达,进而抑制细胞凋亡有关。
AIM： To compare the influence of S.miltiorrhiza Bunge injection（SMBI）and S.przewalskii Maxim injection（SPMI） on H2O2-induced injury in PC12.METHODS： PC12 cells were cultured in vitro to compare the protective effects of SMBI and SPMI on cell injury induced by H2O2.The survival rate was measured by MTT method,and the apoptosis rate of PC12 cells was detected by flow cytometry,the protein expressions of bcl-2 and bax in PC12 cells were detected by immunocytochemistry staining method.RESULTS： When PC12 was damaged by H2O2,the rate of cell survival was degraded,and the rate of apoptosis was increased.SMBI and SPMI could alleviate the destroy effectively（P 〈0.05）,they both could strengthen the expression of bcl-2（P〈0.05） and lighten that of bax（P〈0.05）.CONCLUSION： The results suggested that SMBI and SPMI had comparable protective effect on H2O2-induced injury in vitro.The main effects of both injections were related to strengthening the expression of bcl-2 and lightening that of bax.