文献类型：期刊文献

英文题名：Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Attenuate Persistent Corneal Epithelial Injury by Promoting Retinal Neural-Like Cell Proliferation and Hindering Their Differentiation

作者：Wang, Dengting[1];Wang, Jianyun[2];Sun, Yan[3]

第一作者：Wang, Dengting

通信作者：Sun, Y[1]

机构：[1]Rehabil Ctr Hosp Gansu, Dept Ophthalmol, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Rehabil Ctr Hosp, Dept Adm, Lanzhou 730000, Gansu, Peoples R China;[3]Gansu Univ Chinese Med, Gansu Rehabil Ctr Hosp, Lanzhou Purui Ophthalmol Hosp, Dept Ophthalmol, Lanzhou 730000, Gansu, Peoples R China

第一机构：Rehabil Ctr Hosp Gansu, Dept Ophthalmol, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Univ Chinese Med, Gansu Rehabil Ctr Hosp, Lanzhou Purui Ophthalmol Hosp, Dept Ophthalmol, Lanzhou 730000, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2025

卷号：40

期号：3

起止页码：230

外文期刊名：MOLECULAR GENETICS MICROBIOLOGY AND VIROLOGY

收录：;Scopus(收录号：2-s2.0-105025997392);WOS:【SCI-EXPANDED(收录号:WOS:001649326100010)】；

基金：This work was supported by the Lanzhou Science and Technology Plan Project (no. 2023-3-67).

语种：英文

外文关键词：bone marrow mesenchymal stem cells; exosomes; persistent corneal epithelial injury; human retinal ganglion cells; miR-150-5P; LncRNA MIAT

摘要：Objective: Persistent corneal epithelial defect (PED) is still a challenge to human which is difficultly cured, normally requiring long-term follow-up. Herein, this study aimed at exploring the potential role of bone marrow mesenchymal stem derived exosomes (BMSC-exos) in persistent corneal epithelial injury and its underlying mechanism. Methods: After characterization of BMSC-exos, RGC-5 cell viability was determined by MTT assay and BMSCs surface markers were analyzed by flow cytometry. Additionally, RT-qPCR and Western blot analysis measured miR-150-5p, Brn3, Islet-1, and PCNA expression. Double luciferase assays were conducted to evaluate the targeting relationship between LncRNA-MIAT and miR-150-5p. Results: Presented in ellipsoidal or cup shape, BMSC-exos were positive to BMSCs-specific markers CD29 and CD90, and cell surface markers CD9 and CD63 could be detected on exosomes. Importantly, treatment with BMSC-exos significantly promoted the proliferation of retinal ganglion cells (RGCs) and hindered cell differentiation. Interestingly, down-regulating lncRNA MIAT exerted the same effect as BMSC-exos, increasing cell viability, and decreasing the expression of differentiation-related proteins Brn3 and ISL1. Bioinformatics software predicted miR-150-5P as specific target genes of lncRNA MIAT, and the relative luciferase activity of miR-150-5P+MIAT-WT co-transfection group was lower. Conclusions: BMSC-exos improve PED when targeting and regulating miR-150-5p expression through lncRNA MIAT to up-regulate the expression of PCNA in RGC-5 cells, and down-regulate the expression of Brn3 and ISL1.