文献类型：期刊文献

英文题名：Effect of xanthohumol on Th1/Th2 balance in a breast cancer mouse model

作者：Zhang, Wenchao[1];Pan, Yanlong[1,2];Gou, Panhong[3];Zhou, Cheng[1];Ma, Lianqing[4];Liu, Qiming[4];Du, Yuping[1];Yang, Jinbo[1];Wang, Qin[1]

第一作者：Zhang, Wenchao

通信作者：Wang, Q[1]

机构：[1]Lanzhou Univ, Sch Life Sci, Inst Canc Biol & Drug Screening, 222 Tianshui South Rd, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Ctr Sci Expt, Lanzhou 730000, Gansu, Peoples R China;[3]Lanzhou Univ, Sch Publ Hlth, Inst Toxicol, Lanzhou 730000, Gansu, Peoples R China;[4]Yumen Tuopu Sci & Technol Dev Co Ltd, Yumen 735211, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Life Sci, Inst Canc Biol & Drug Screening, 222 Tianshui South Rd, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Life Sci, Inst Canc Biol & Drug Screening, 222 Tianshui South Rd, Lanzhou 730000, Gansu, Peoples R China.

年份：2018

卷号：39

期号：1

起止页码：280

外文期刊名：ONCOLOGY REPORTS

收录：;Scopus(收录号：2-s2.0-85037073090);WOS:【SCI-EXPANDED(收录号:WOS:000418358200030)】；

基金：This study was supported by the Science and Technology Support Projects of Gansu Province (no. 1104FKCA123; http://www.gsstc.gov.cn). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：xanthohumol; antitumor immunity; CTL responses; Th1/Th2 balance; STAT4

摘要：Xanthohumol (XN), a prenylflavonoid found in the hop plant, Humulus lupulus, exhibits a variety of biological activities. Numerous studies have reported that XN inhibits the growth of many types of cancer cells, but the effects of XN on tumor immunity have not yet been studied. We explored the effect of XN on Th1/Th2 balance and the underlying mechanism based on a BALB/c-4T1 breast cancer mouse model. The results showed that XN significantly slowed down tumor growth and inhibited expression of antitumor proliferation protein Ki-67 as well as breast cancer-specific marker cancer antigen 15-3 (CA15-3). Flow cytometric analysis revealed that XN enhanced the secretion of perforin, granzyme B and increased the ratio of CD8(+)/CD25(+). ELISA analysis of cytokine results demonstrated that XN obviously upregulated Th1 cytokines, while downregulated Th2 cytokines. Th1/Th2 ratio analysis by flow cytometry illustrated that XN regulated the balance drift to Th1 polarization. Western blotting and immunohistochemistry (IHC) results manifested that XN induced expression of T-bet, a Th1-specific transcription factor. Furthermore, we found that XN significantly promoted the phosphorylation of signal transducer and activator of transcription (STAT)4. Our results demonstrated that XN promoted Th1/Th2 balance towards Th1 polarization, and STAT4 may play a positive role in the regulation of Th1/Th2 cytokines by XN.