文献类型：期刊文献

中文题名：黄芪多糖对IL-6和TNF-α模拟炎性微环境中BMSCs增殖及TAFs分化的影响

英文题名：Effects of astragalus polysaccharide on BMSCs proliferation and TAFs differentiation in inflammatory microenvironment simulated by IL-6 and TNF-α

作者：李程豪[1];任春贞[1];刘永琦[1,2];骆亚莉[1]

第一作者：李程豪

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]敦煌医学与转化省部共建教育部重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2018

卷号：33

期号：7

起止页码：3039

中文期刊名：中华中医药杂志

外文期刊名：China Journal of Traditional Chinese Medicine and Pharmacy

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2017_2018】；

基金：国家自然科学基金项目(No.81360588)~~

语种：中文

中文关键词：黄芪多糖;骨髓间充质干细胞;炎性微环境;增殖;分化;肿瘤相关成纤维细胞

外文关键词：Astragalus polysaccharides;Bone marrow mesenchymal stem cells;Inflammatory microenvironment;Proliferation;Differentiation;Tumor associated fibroblasts

摘要：目的:探讨黄芪多糖对炎性微环境中骨髓间充质干细胞(BMSCs)增殖、分化及肿瘤相关成纤维细胞(TAFs)相关分子表达的影响。方法:采用IL-6和TNF-α模拟BMSCs体外炎性微环境培养体系,并以黄芪多糖最佳药物浓度干预该体系;通过CCK-8法、流式细胞术及Western Blot分别检测黄芪多糖对炎性微环境中BMSCs细胞增殖、周期及TAFs标记分子α-SMA、FAP蛋白的表达变化。结果:与正常对照组比较,炎性因子干预组细胞生长速度加快,G1期比例降低,S期比例升高,且α-SMA、FAP蛋白表达明显增加,均有显著性差异(P<0.05);与炎性因子干预组比较,黄芪多糖组(50mg/L)生长速度减慢,G1期比例升高,S期比例降低,且α-SMA、FAP蛋白表达有所降低(P<0.05)。结论:IL-6和TNF-α模拟炎性微环境中BMSCs形态、增殖特性发生异常改变,具有向肿瘤相关成纤维细胞分化性,且黄芪多糖可抑制炎性微微环境中BMSCs异常改变。
Objective： To study the effects of astragalus polysaccharide on bone marrow mesenchymal stem cells（BMSCs） proliferation, differentiation and tumor associated fibroblasts molecular expression under inflammatory microenvironment. Methods： Interleukin 6（IL-6） and tumor necrosis factor-α（TNF-α） were used to simulate BMSCs in vitro inflammatory microenvironment, and optimal concentration of astragalus polysaccharide was used for intervention of this system. Effects of astragalus polysaccharide on BMSCs proliferation, cycle as well as protein expression of biomarkers of TAFs, α-SMA and FAP were detected respectively by CCK-8 method, flow cytometry and Western Blot. Results： Compared with normal control group, inflammatory factor intervention group showed accelerated cell growth speed, reduced G1 phase and increased S phase proportion, and α-SMA, FAP protein expression significantly increased（P〈0.05）. Compared with inflammatory factor intervention group, astragalus polysaccharide group（50 mg/L） showed slow growth speed, increased G1 phase and reduced S phase proportion, and α-SMA, FAP protein expression decreased（P〈0.05）. Conclusion： There are abnormal changes of BMSCs morphology and proliferation characteristics, which was likely to differentiate into tumor related fibroblasts, and astragalus polysaccharide can inhibit abnormal changes of BMSCs in the inflammatory environment.