文献类型：期刊文献

英文题名：Plasma miR-1273g-3p acts as a potential biomarker for early Breast Ductal Cancer diagnosis

作者：Guo, Huan[1,2];Zeng, Xiangting[3];Li, Haining[2];Guo, Yanxiang[2];Wang, Tao[2];Guo, Hongyun[2];Zhu, Gongjian[2];Wang, Lan[2];Zhou, Haihong[2];Liu, Kedan[2];Chen, Xiaoyi[2];Wang, Haitao[2];Zhao, Xinke[4];Su, Haixiang[2,4];Li, Yingdong[1,4]

第一作者：Guo, Huan

通信作者：Li, YD[1];Su, HX[2];Su, HX[3];Li, YD[3]

机构：[1]Lanzhou Univ, Sch Basic Med Sci, Lanzhou, Gansu, Peoples R China;[2]Gansu Prov Canc Hosp, Gansu Prov Acad Inst Med Sci, Lanzhou, Gansu, Peoples R China;[3]Lanzhou Univ, Dept Gen Surg, Hosp 2, Lanzhou, Gansu, Peoples R China;[4]Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China

第一机构：Lanzhou Univ, Sch Basic Med Sci, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Sch Basic Med Sci, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Gansu Prov Canc Hosp, Gansu Prov Acad Inst Med Sci, Lanzhou, Gansu, Peoples R China;[3]corresponding author), Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China.|[10735]甘肃中医药大学;

年份：2020

卷号：92

期号：1

起止页码：1

外文期刊名：ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS

收录：;Scopus(收录号：2-s2.0-85086474780);WOS:【SCI-EXPANDED(收录号:WOS:000529347300001)】；

基金：This study was supported by a grant from Scientific Research Project of Health and Family Planning Commission of Gansu Province, China (Grant No.GSWSKY-2015-25). This research was supported by the Natural Science Foundation for Young Scientists through Gansu provincial Science and Technology Department, China (Grant No.17JR5RA042& No.1606RJYA296). This research was supported by Gansu science and technology program (Grant No.17JR3TA013) through Gansu provincial Science and Technology Department, China. The authors thank the patients and the healthy donors who participated in this study.

语种：英文

外文关键词：breast ductal carcinoma; plasma; miR-1273g-3p; circulating miRNA; biomarker

摘要：Circulating miRNAs presenting in plasma in a stable manner have been demonstrated their potential role as a promising biomarkers in many human diseases, such as Alzheimer's disease, melanoma and ovarian carcinoma. However, few circulating miRNAs could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p as a biomarker for detecting breast ductal cancer. We detected miR-1273g-3p levels in the plasma of 39 sporadic breast ductal cancer patients and 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed the plasma miR-1273g-3p level were significantly up-regulated in breast ductal cancer patients compared with healthy donors (p=0.0139). Receiver operating characteristic (ROC) curve also revealed the significantly diagnostic ability of miR-1273g-3p in patients (p=0.0414). In addition, the plasma level of miR-1273g-3p was closely related to IIIB-IIIC TNM stage. We also confirmed the higher expression level of miR-1273g-3p in breast cancer cell lines MCF-7 (4.872 +/- 0.537) than normal breast cells (Hs 578Bst). Taken together, miR-1273g-3p could represent as a potential biomarker for early breast ductal cancer diagnosis.