文献类型：期刊文献

英文题名：Prenatal ethanol exposure-induced hypothalamic an imbalance of glutamatergic/GABAergic projections and low functional expression in male offspring rats

作者：Lu, Juan[1,4];Li, Qiang[4];Ma, Guoqin[4];Hong, Chenghao[1];Zhang, Wenqian[1];Wang, Yuxia[1];Wang, Hui[2,3]

第一作者：Lu, Juan

通信作者：Wang, YX[1];Wang, H[2]

机构：[1]Jinan Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Guangzhou 510630, Guangdong, Peoples R China;[2]Wuhan Univ, Dept Pharmacol, Sch Basic Med Sci, Wuhan 430071, Peoples R China;[3]Hubei Prov Key Lab Dev Originated Dis, Wuhan 430071, Peoples R China;[4]Gansu Univ Chinese Med, Dept Orthoped, Gansu Prov Hosp TCM, Lanzhou 730050, Gansu, Peoples R China

第一机构：Jinan Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Guangzhou 510630, Guangdong, Peoples R China

通信机构：[1]corresponding author), Jinan Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Guangzhou 510630, Guangdong, Peoples R China;[2]corresponding author), Wuhan Univ, Dept Pharmacol, Basic Med Sch, Wuhan 430071, Hubei, Peoples R China.

年份：2020

卷号：141

外文期刊名：FOOD AND CHEMICAL TOXICOLOGY

收录：;Scopus(收录号：2-s2.0-85085578380);WOS:【SCI-EXPANDED(收录号:WOS:000541110800041)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (81660544, 81901468 and 81673524), the National Key Research and Development Programme of China (No. 2017YFC1001300), the Major Technological Innovation Projects of Hubei Province (2019ACA140), the Guangzhou Science and Technology Projects (201707010265) and the Medical Science Advancement Program (Basic Medical Sciences) of Wuhan University (TFJC2018001).

语种：英文

外文关键词：Low functional expression; Hypothalamus; Ethanol; Glutamatergic/GABAergic projections; Developmental programming; Glucocorticoid metabolic activation system

摘要：This study was designed to demonstrate that prenatal ethanol exposure (PEE) can induce low functional expression of the hypothalamus in male offspring rats and explore the underlying mechanism. Pregnant rats were administered 4 g/kg ethanol or normal saline by oral gavage each day from gestational day (GD) 9 to GD20. Male GD20 foetuses and postnatal day 120 adult offspring rats were sacrificed under anaesthesia. Hypothalamic cells from male GD20 similar to postnatal day (PD) 7 rats were treated with different doses of corticosterone and the glucocorticoid receptor (GR) antagonist mifepristone for 5 days. In this study, we found that PEE-induced overexposure of maternal glucocorticoids enhanced the expression of L-glutamic acid decarboxylase (GAD) 67 in the hypothalamic paraventricular nucleus (PVN) by activating the glucocorticoid metabolic activation system, further inducing the conversion of glutamate to L-gamma-aminobutyric acid (GABA) and developmental imbalance of glutamatergic/GABAergic projections to the PVN. The imbalance change was maintained until after birth, resulting in the inhibition of parvocellular neurons and low functional expression of the hypothalamus in PEE offspring rats. Our study indicated that low functional expression of the hypothalamus in male PEE offspring rats was associated with developmental programming of an imbalance of glutamatergic/GABAergic projections to the PVN.