文献类型：期刊文献

中文题名：miR-20a-5p/miR-20b-5p在胃癌细胞和组织中的表达及调控靶基因信号通路富集分析

英文题名：Expressions of miR-20a-5p/miR-20b-5p in gastric cancer cells and gastric carcinoma tissues and enriching analysis of their target genes and signal pathways

作者：李海龙[1,2];宋耀辉[1];陈兆峰[3];王晶[1,2];田一虹[1,2];王圆圆[1,2];陈彻[1];牛媛瑕[4]

第一作者：李海龙

机构：[1]甘肃中医药大学临床医学院临检基础教研室,兰州730000;[2]甘肃省中医方药挖掘与创新转化重点实验室,甘肃省中药新产品创制工程实验室,兰州730000;[3]兰州大学第一医院消化科,兰州730000;[4]甘肃中医药大学附属医院检验科,兰州730000

第一机构：甘肃中医药大学临床医学院|甘肃中医药大学中医临床学院

年份：2017

卷号：35

期号：11

起止页码：822

中文期刊名：临床检验杂志

外文期刊名：Chinese Journal of Clinical Laboratory Science

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：甘肃省高等学校科研项目(甘财教[2014]63-15)

语种：中文

中文关键词：miR-20a-5p;miR-20b-5p;胃癌

外文关键词：miR-20a-5p; miR-20b-5p; gastric cancer

摘要：目的观察miR-20a-5p/miR-20b-5p在胃癌细胞和胃癌组织中的表达,并用生物信息学方法分析其靶基因和富集的信号通路,探讨其生物学行为和功能。方法采用实时荧光定量PCR法检测miR-20a-5p/miR-20b-5p在不同分化程度(高、中、低)的胃癌细胞和正常胃黏膜细胞以及其在胃癌组织和癌旁组织中的表达水平,并分析其临床意义,采用mir WALK网站数据库的预测软件预测其靶基因,选择该网站10个软件中具备3个以上软件支持的靶基因,用DAVID 6.7在线富集其靶基因参与的信号通路。结果 miR-20a-5p/miR-20b-5p在不同分化程度(高、中、低)的胃癌细胞中表达上调(P均<0.05),胃癌组织相对于癌旁组织亦明显上调表达(P<0.05),miR-20b-5p表达上调与胃癌淋巴结侵袭转移以及浸润深度关系密切(P均<0.05);生物信息学分析提示,miR-20a-5p/miR-20b-5p的靶基因富集存在于肿瘤相关的多个信号通路中。结论 miR-20a-5p/miR-20b-5p在胃癌细胞系及组织中呈高表达,并于与淋巴结侵袭转移以及浸润深度有关,可作为胃癌潜在的生物学标志物。
Objective To investigate the expressions of miR-20 a-5 p/miR-20 b-5 p in gastric cancer cells and gastric carcinoma tissues,analyze their target genes and enriching signal pathways using bioinformatics methods,and explore their biological behavior and function. Methods The expression levels of miR-20 a-5 p/miR-20 b-5 p in gastric cancer cells with different differentiation such as high,middle or low differentiation,normal gastric mucosa cells,gastric cancer tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR,and their clinical significance was analyzed. The target genes of miR-20 a-5 p/miR-20 b-5 p were predicted using 10 softwares affiliated to mir WALK web database,and the genes supported by more than three softwares were selected as target genes. The signal pathways of target genes were enriched by online DAVID 6. 7 software. Results The expression levels of miR-20 a-5 p/miR-20 b-5 p in gastric cancer cells with different differentiation were significantly higher than that in normal gastric mucosa cells（ all P〈0. 05）,and that in gastric cancer tissues higher than adjacent tissues（ P〈0. 05）. The up-regulated expression of miR-20 b-5 p was closely related to lymph node metastasis and invasion depth（ all P〈0. 05）. Bioinformatics analysis showed that the enriched target genes of miR-20 a-5 p/miR-20 b-5 p existed in multiple signaling pathways associated with cancer. Conclusion MiR-20 a-5 p/miR-20 b-5 p may be a promising biomarker of gastric cancer,which is highly expressed in gastric cancer and is related to lymph node metastasis and invasion depth.