文献类型：期刊文献

英文题名：Semi-bionic extraction of compound turmeric protects against dextran sulfate sodium-induced acute enteritis in rats

作者：Wang, Ruiqiong[1];Wu, Guotai[2];Du, Lidong[2];Shao, Jing[2];Liu, Fenglin[2];Yang, Zhijun[2];Liu, Dongling[2];Wei, Yanming[1]

第一作者：Wang, Ruiqiong

通信作者：Wei, YM[1]

机构：[1]Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Key Lab Pharmacol & Toxicol Tradit Chinese Med Ga, Lanzhou 730000, Gansu, Peoples R China

第一机构：Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Gansu, Peoples R China

通信机构：[1]corresponding author), Gansu Agr Univ, Coll Vet Med, Lanzhou 730070, Gansu, Peoples R China.

年份：2016

卷号：190

起止页码：288

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;Scopus(收录号：2-s2.0-84975090066);WOS:【SCI-EXPANDED(收录号:WOS:000383293300027)】；

基金：This study was supported by the National Natural Science Foundation of China (No.31272600) and the Program of Gansu Province Key Laboratory of Pharmacology and Toxicology of TCM (No. ZDSYS-KJ-2013-006). The authors are grateful to Dr. Changtian Li and Dr. Qiulan Wang for providing technical assistance in pathological observations and to Dr. Hailong Li for English language revisions.

语种：英文

外文关键词：Compound turmeric; Semi-bionic extraction; Acute enteritis; Anti-inflammatory; Antioxidant

摘要：Ethnopharmacological relevance: Compound turmeric has been widely used as a remedy for infectious diseases in China. It is a classic multi-herb prescription in traditional Chinese medicine, commonly used in the treatment of enteritis, pneumonia, and abdominal pain for hundreds of years. However, throughout this history, the powder of multi-herbs was directly swallowed, which is currently difficult to administer to patients. The extract of Chinese herbal medicine is made by semi-bionic extraction technology, which is great progress in the modernization of powders of traditional Chinese medicine. The aim of this work is to investigate the protective effects of semi-bionic extraction of compound turmeric (SET) on acute enteritis (AE) induced by dextran sulfate sodium (DSS) in rats. Materials and methods: SET was extracted in artificial gastric juice or artificial intestinal juice and mixed. After vacuum drying, the SET powder was dissolved in distilled water. Adult male Sprague Dawley rats were randomly divided into six groups. Rats were given salazosulfapyridine (SASP, 175.0 mg/kg) or SET (0.42 or 0.21 g/kg) before intragastric administration of 5% DSS solutions (0.75 g/kg). The treatments lasted 7 days. The food intake in 24 h, disease activity index (DAI), and wet/dry (W/D) weight ratios and histological changes in colon tissue were measured. The tumor necrosis factor-alpha (TNIF-alpha), interleukin-6 (IL-6), IL-1 beta, IL-8, and IL-10 in serum were determined at 1, 4, or 7 d after DSS challenge. Myeloperoxidase (MPO), malonaldehyde (MDA), diamine oxidase (DAO), and glutathione peroxidase (GSH-Px) activities in colon tissue were determined at 7 d. In addition, the nuclear factor-kappa (NF-kappa B) and intercellular cell adhesion molecule-1 (ICAM-1) activations in colon tissue were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. Results: In rats with AE, SET significantly reduced DAI at 7 d after DSS treatment, increased the body weight of rats and the food intake in 24 h at 3 or 6 d after DSS challenge, and reduced the colon W/D ratio. SET also reduced the TNF-alpha, IL-6, IL-1 beta, and IL-8 in serum and increased IL-10 in serum at 4 and 7 d. In addition, SET decreased MPO, MDA, DAO, and GSH-Px activities in colon and attenuated histological changes in the colon at 7 d after DSS treatment. Further studies demonstrated that SET significantly inhibited NF-icB and ICAM-1 activations in colon tissue. Conclusions: The current study demonstrated that SET has potent protective effects on DSS-induced AE in rats through its anti-inflammatory and anti-oxidant activities. (C) 2016 Elsevier Ireland Ltd. All rights reserved.