文献类型：期刊文献

中文题名：水蛭素对博莱霉素致特发性肺间质纤维化大鼠肺组织中AKT、p-AKT蛋白表达的影响

英文题名：The effect of hirudin on level of AKT and p-AKT protein in lung tissue of rats with idiopathic pulmonary fibrosis induced by bleomycin

作者：张景容[1];谢海彬[2];沈明霞[2];王海霞[1];高绒霞[1];马玉坤[1];李红[3]

第一作者：张景容

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730000;[3]深圳市罗湖区中医院,广东深圳518001

第一机构：甘肃中医药大学

年份：2019

卷号：11

期号：11

起止页码：5

中文期刊名：中医临床研究

外文期刊名：Clinical Journal Of Chinese Medicine

基金：甘肃省科技厅自然基金(1308RJZA197)

语种：中文

中文关键词：水蛭素;特发性肺间质纤维化;AKT;p-AKT

外文关键词：Hirudin;Idiopathic pulmonary fibrosis;AKT;p-AKT

摘要：目的:本文以水蛭素为研究药物,通过Western blot法测定蛋白激酶B(AKT)、p-AKT蛋白在肺中的表达,分析水蛭素对特发性肺间质纤维化(Idiopathic Pulmonary Fibrosis,IPF)的干预效果及作用机制,为水蛭素的临床运用奠定基础,并拓宽IPF的治疗途径。方法:选取SPF级Wistar大鼠180只,随机选18只为空白组(K组),剩余大鼠统一造模(气管内注射博莱霉素),选取108只造模成功大鼠,随机分为模型组(M组)、激素组(J组)、低剂量组(D组)、高剂量组(G组)、中剂量组(Z组)、中剂量联合激素组(ZJ组),每组18只,初次给药为造模后第2天,在给药的第7d、14d、28d分批处死取材,检测AKT、p-AKT蛋白的表达。结果:AKT蛋白表达量:K组在各时间点AKT蛋白表达量均最低,M组在7d、28d含量明显高于J组(P<0.01);7d时G组低于J组(P<0.01),14、28d时G、ZJ组均低于J组(P<0.01);7d、28d时D、Z组高于J组(P<0.01)。p-AKT蛋白表达量:各时间点K组最低,M组最高;J组低于M组(P<0.01);ZJ组低于J组(P<0.01);7d、14d时G组低于J组(P<0.01);28d时G组高于J组,但P>0.05。结论:IPF发病过程中,PI3K/AKT信号通路中AKT、p-AKT蛋白表达明显升高,说明该信号通路与IPF发病过程有明显相关性;水蛭素能降低AKT、p-AKT蛋白的表达量,减缓IPF病程发展;水蛭素极有可能通过PI3K/AKT通路作用于IPF。
Objective:Hirudin as a research drug,the expression of AKT and p-AKT protein in the lung were determined by Western blot.The intervention effect and mechanism of hirudin on IPF were analyzed,which laid a foundation for the clinical application of hirudin and broadened the therapeutic approach of IPF.Methods:180 SPF-level Wistar rats were selected.18 rats were randomly selected as the blank group (K group).The remaining rats were uniformly modeled (intratracheal injection of bleomycin);108 rats were randomly divided into the model group (M group),hormone group (J group),hirudin low dose group (D group),high dose group (G group),medium dose group (Z group),and middle dose plus hormone group (ZJ group),18 rats in each group.On the 2 days after modeling,rats were given first dose medicine.The samples were killed in batches on the 7th,14th,and 28th day after administration,and the expression of AKT and p-AKT protein were detected.Results:The expression of AKT protein was the lowest in K group at each time point;at 7 days and 28 days, that in M group was significantly higher than J group (P<0.01);at 7 days,that in G group was lower than J group (P<0.01);at the 14th and 28th day,that in G group and ZJ group was lower than J group (P<0.01);at 7 days and 28 days,that in the D group and Z group was higher than J group (P<0.01).The p-AKT protein expression was the lowest in K group and the highest in M group at each time point;that in J group was lower than M group (P<0.01);that in ZJ group was lower than J group (P<0.01);that in G group was lower than J group at 7 day and 14 day (P<0.01);at 28 days,that in G group was higher than J group,P>0.05.Conclusion:During the pathogenesis of IPF,the expression of AKT and p-AKT protein in PI3K/AKT signaling pathway was significantly increased,indicating that the signaling pathway is significantly correlated with the pathogenesis of IPF.Hirudin can reduce the expression of AKT and p-AKT protein and slow down The progression of IPF disease;hirudin is highly likely to act on IPF through the PI3K/AKT pathway.