文献类型：期刊文献

英文题名：Multi-Omics Analysis of the Dual Role of PIP5K1B in Gastric Adenocarcinoma: Regulation of Myofibroblasts, Remodeling of the Immune Microenvironment, and Novel Strategies for Precision Treatment

作者：Liang, Tong[1];Cui, Yaqiong[2]

第一作者：梁恬;梁婷

通信作者：Cui, YQ[1]

机构：[1]Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Prov Hosp, Dept Radiol, Lanzhou 730000, Gansu, Peoples R China

第一机构：甘肃中医药大学

通信机构：[1]corresponding author), Gansu Prov Hosp, Dept Radiol, Lanzhou 730000, Gansu, Peoples R China.

年份：2026

外文期刊名：CURRENT MEDICINAL CHEMISTRY

收录：;Scopus(收录号：2-s2.0-105044141523);WOS:【SCI-EXPANDED(收录号:WOS:001816818200001)】；

基金：This work was supported by the Intramural Research Fund Project of Gansu Provincial Hospital (23GSSYF-5) and the Gansu University of Chinese Medicine Talent Recruitment Program (2026YJRC-10).

语种：英文

外文关键词：stomach adenocarcinoma; pan-cancer; biomarker; tumor microenvironment

摘要：Objective Phosphatidylinositol-4-phosphate 5-kinase type 1 beta (PIP5K1B), an enzyme linked to actin cytoskeleton dynamics, has a paradoxical role in cancer. While pan-cancer analyses show its dual nature, its impact on overall survival (OS) in stomach adenocarcinoma (STAD) is unclear. Methods We combined bulk RNA sequencing data (TCGA/GTEx, 33 cancers) and single-cell transcriptomics data (STAD cohort) to track PIP5K1B expression in STAD. Cox regression and Kaplan-Meier models assessed their prognostic effect on OS, with immune infiltration and methylation-epigenetic correlation analyses. GDSC pharmacogenomic data evaluated drug sensitivity links. Results Pan-cancer analysis found PIP5K1B had a unique prognostic impact in STAD, with high expression raising death risk (OS: hazard ratios (HR) = 1.55, 95% confidence interval [CI] = 1.31 - 1.84, P = 3.5e-07). Single-cell mapping showed PIP5K1B in tumor-associated myofibroblasts, regulating tumor cell antigen processing via toll-like receptor signaling. Clinically, STAD patients had higher PIP5K1B expression (P = 0.0013), and death risk rose with cancer stage (HR = 2.375, 95% CI = 1.499 - 3.762, P = 0.00058). Despite immune evasion in high-PIP5K1B tumors, they were sensitive to bleomycin (P < 0.001, R-2 = 0.06) and docetaxel (P < 0.001, R-2 = 0.21). Discussion The study's findings underscore the potential of PIP5K1B as a prognostic biomarker and therapeutic target in gastric cancer, opening up new possibilities for personalized treatment approaches. Conclusion Integrating pan-cancer multi-omics data, we found PIP5K1B serves as a significant prognostic biomarker in STAD, with its high expression linked to poor survival outcomes. Its expression in tumor - associated myofibroblasts and involvement in antigen processing suggest a complex role in the tumor immune landscape. Furthermore, the sensitivity of high - PIP5K1B tumors to specific chemotherapies offers new avenues for targeted treatment strategies in STAD.