文献类型：期刊文献

中文题名：掌叶大黄多糖超滤工艺优选

英文题名：Optimization of Ultrafiltration Technology for Polysaccharides from Rheum palmatum

作者：李芸[1];苗小楼[2];魏舒畅[1];吴平安[1];刘峰林[1];李秀娟[3]

第一作者：李芸

机构：[1]甘肃中医学院甘肃省高校中藏药化学与质量研究省级重点实验室,兰州730000;[2]中国农业科学院兰州畜牧与兽药研究所,兰州730050;[3]甘肃省中医院,兰州730050

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2012

卷号：18

期号：18

起止页码：55

中文期刊名：中国实验方剂学杂志

外文期刊名：Chinese Journal of Experimental Traditional Medical Formulae

收录：CSTPCD;;北大核心:【北大核心2011】；

基金：甘肃省教育厅第二批科研项目(0806B-07)

语种：中文

中文关键词：掌叶大黄多糖;超滤;纯化工艺

外文关键词：polysaccharides from Rheum palmatum; ultra-filtration; purification process

摘要：目的:探讨超滤膜技术分离纯化掌叶大黄多糖的可行性工艺。方法:采用正交设计法优选超滤前离心条件;以超滤压力、料液温度及料液浓度为考察因素,以粗多糖得率和含量为考核指标,均匀设计优选超滤工艺参数。结果:最佳超滤条件为药液以3 000 r.min-1离心处理12 min,用分子截留量10万的膜,超滤压力为0.05～0.09 MPa,药液温度40℃,药材与提取液质量比1∶10～1∶30,pH 6.0,超滤膜先用tween-80(5 g.L-1)处理10 min。结论:优化所得超滤工艺与未超滤相比有较高的多糖得率和含量。超滤技术可用于纯化、富集掌叶大黄多糖,具有产业化开发前景。
Objective： To study on separation and purification feasible process of polysaccharides from R. palmatum by uhrafiltration. Method： Centrifugation conditions were optimized by orthogonal test before ultrafiltration, ultrafiltration technology parameters were optimized by uniform design with yield and concent of polysaccharides as indexes, and ultrafiltration pressure, liquid temperature and liquid concentration were selected as investigated factors. Result： Optimum ultra-filtration process was： centrifuged 12 rain with 3 000 r ·min^（-1）, membrane with 105 molecular interception, ultrafiltration pressure 0.05-0.09 MPa, liquid temperature 40 ℃ , ratio of solid-liquid 1 ： 10-1 ： 30, pH 6.0, uhrafiltration membrane was pretreated 10 min with tween-80 （5 g· L^（-1） ）. Conclusion： Optimized ultrafiltration process could obtain higher yield and concent of polysaccharides while compared with non-ultrafihration. Uhrafiltration technology could be used for purification and enrichment of polysaccharides from R. palmatum with industrial development prospect.