文献类型：期刊文献

英文题名：Time-course metabolic profiling in alfalfa leaves under Phoma medicaginis infection

作者：Fan, Qin[1,2];Creamer, Rebecca[3];Li, Yanzhong[1,4]

第一作者：樊秦;Fan, Qin

通信作者：Li, YZ[1];Li, YZ[2]

机构：[1]Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst,Key Lab Grass, Engn Res Ctr Grassland Ind,Minist Educ,Minist Min, Lanzhou, Gansu, Peoples R China;[2]Gansu Univ Chinese Med, Lanzhou, Gansu, Peoples R China;[3]New Mexico State Univ, Las Cruces, NM 88003 USA;[4]Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China

第一机构：Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst,Key Lab Grass, Engn Res Ctr Grassland Ind,Minist Educ,Minist Min, Lanzhou, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Coll Pastoral Agr Sci & Technol, State Key Lab Grassland Agroecosyst,Key Lab Grass, Engn Res Ctr Grassland Ind,Minist Educ,Minist Min, Lanzhou, Gansu, Peoples R China;[2]corresponding author), Chinese Acad Agr Sci, Inst Grassland Res, Hohhot, Peoples R China.

年份：2018

卷号：13

期号：10

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000448641200052)】；

基金：This research was financially supported by Special Fund for Agro-scientific Research in the Public Interest (No. 201303057) to Yanzhong Li. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：Information on disease process and pathogenicity mechanisms is important for understanding plant disease. Spring black stem and leaf spot caused by the necrotrophic pathogen Phoma medicaginis var. medicaginis Malbr. & Roum causes large losses to alfalfa. However, till now, little is known about alfalfa-P. medicagnis interactions and the pathogenicity mechanisms of the pathogen. Here, alfalfa inoculated with P. medicaginis was subjected to GC-MS based metabolic profiling. The metabolic response in P. medicaginis-inoculated and mock-inoculated alfalfa leaves was assessed at 2, 4, 6, 8, 12, 16, 20, 24, 26 and 28 days post inoculation. In total, 101 peaks were detected in the control and inoculated groups, from which 70 metabolites were tentatively identified. Using multivariate analysis, 16 significantly regulated compounds, including amino acids, nitrogen-containing compounds and organic acids, polyols, fatty acids, and sugars were tentatively identified (Variable importance values, VIP>1.0 and p <0.05). Among these metabolites, the levels of malate, 5-oxoproline, palmitic acid and stearic acid were increased significantly in P. medicaginis-infected alfalfa leaves compared to the controls. In contrast, the levels ofy-aminobutyric acid and 2-pyrrolidinone were significantly decreased in infected leaves compared to the controls. Further metabolic pathway analysis of the 16 significantly regulated compounds demonstrated that glycolysis, the tricarboxylic acid cycle, and beta-oxidation of fatty acids were significantly induced in the alfalfa leaves at later stages of P. medicaginis infection. The strong induction of tricarboxylic acid cycle pathways at later infection stages caused by the pathogen may induce senescence in the alfalfa leaves, leading to plant death. However, intermediate metabolites of these metabolic pathways, and inositol phosphate, glutathione, the metabolic pathways of some amino acids accumulated rapidly and strongly at early stages of infection, which may enhance the ability of alfalfa to resist necrotrophic P. medicaginis disease. Understanding metabolic pathways is essential for understanding pathogenesis.