文献类型：期刊文献

英文题名：Quercetin Attenuates Visceral Hypersensitivity and 5-Hydroxytryptamine Availability in Postinflammatory Irritable Bowel Syndrome Rats: Role of Enterochromaffin Cells in the Colon

作者：Qin, Hong-Yan[1];Zang, Kai-Hong[2];Zuo, Xiao[1,3];Wu, Xin-An[1];Bian, Zhao-Xiang[4]

第一作者：Qin, Hong-Yan

通信作者：Qin, HY[1]

机构：[1]Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Gansu, Peoples R China;[2]Gansu Univ Tradit Chinese Med, Coll Pharm, Lanzhou, Gansu, Peoples R China;[3]Lanzhou Univ, Sch Pharmaceut, Lanzhou, Gansu, Peoples R China;[4]Hong Kong Baptist Univ, Sch Chinese Med, Lab Brain & Gut Res, Hong Kong, Peoples R China

第一机构：Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Gansu, Peoples R China

通信机构：[1]corresponding author), Lanzhou Univ, Hosp 1, Dept Pharm, Lanzhou 730000, Gansu, Peoples R China.

年份：2019

卷号：22

期号：7

起止页码：663

外文期刊名：JOURNAL OF MEDICINAL FOOD

收录：;Scopus(收录号：2-s2.0-85068866031);WOS:【SCI-EXPANDED(收录号:WOS:000462594200001)】；

基金：This work was supported by the National Science Foundation of China (81341145 and 81400596); the Natural Science Foundation of Gansu Province (grant number 1606RJZA117), and the Fundamental Research Funds for the Central Universities (lzujbky-2014-223 and lzujbky-2015-38).

语种：英文

外文关键词：5-HT availability; enterochromaffin cells; enteroendocrine cells; post-inflammatory irritable bowel syndrome; quercetin

摘要：Intestinal enterochromaffin (EC) cell hyperplasia and increased 5-hydroxytryptamine (5-HT) availability play key roles in the pathogenesis of abdominal hypersensitivity of irritable bowel syndrome (IBS). This study aims to study the effect of quercetin on visceral pain and 5-HT availability in postinflammatory IBS (PI-IBS) rats. PI-IBS model rats were administered quercetin by gavage at doses of 5, 10, and 20 mg/kg for 14 days. Compared with normal rats, the visceral pain threshold of PI-IBS rats was markedly decreased and the abdominal motor response to colon distension was markedly increased. The EC cell count and 5-HT level, as well as tryptophan hydroxylase (TPH) protein, were all significantly elevated in PI-IBS rats, while the 5-HT reuptake transporter (serotonin transporter) was reduced. Genes that are responsible for enteroendocrine cell differentiation, that is, Ngn3 and pdx1, were significantly increased in the PI-IBS group. Quercetin treatment markedly elevated the pain threshold pressure and decreased the visceral motor response of PI-IBS animals; and EC cell density and 5-HT level, as well as TPH expression, in the PI-IBS group were all reduced by quercetin. Quercetin treatment also significantly reduced colonic expression of Ngn3 and pdx1 of PI-IBS. Findings from the present study indicated that the analgesic effect of quercetin on PI-IBS may result from reduction of 5-HT availability in the colon, and the regulatory role of quercetin in endocrine progenitors may contribute to reduced EC cells.