文献类型：期刊文献

中文题名：黄芪百合颗粒对高原低氧模型小鼠肠黏膜屏障的保护作用

英文题名：Protective effects of Astragalus-Lily granules on intestinal mucosal barrier of mice in high altitude hypoxia

作者：李玲[1];安方玉[1];刘永琦[1,2];骆亚莉[1];伍志伟[1];王凤梅[1];任春贞[1];卢志伟[1];苏韫[1]

第一作者：李玲

机构：[1]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,兰州730000;[2]甘肃中医药大学敦煌医学与转化省部共建教育部重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2016

卷号：41

期号：9

起止页码：773

中文期刊名：解放军医学杂志

外文期刊名：Medical Journal of Chinese People's Liberation Army

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：2012甘肃省科技支撑计划项目(120FKCA169);陇原青年创新人才扶持计划(2014-93-72)~~

语种：中文

中文关键词：黄芪百合颗粒;高原低氧;缺氧诱导因子1,α亚基;肠道菌群

外文关键词：Astragalus-Lily granules; high altitude hypoxia; hypoxia-inducible factor 1α subunit; intestinal flora

摘要：目的观察黄芪百合颗粒对高原低氧环境下小鼠肠黏膜及肠道菌群稳态的保护作用。方法使用高压氧舱建立高原低氧模型小鼠。60只昆明小鼠随机分为空白组、模型组及黄芪百合颗粒制剂低、中、高剂量[1.75、3.5、7g/(kg·d)]组。常规饲养3d后灌胃给药,空白组、模型组给予等量双蒸水,1次/d,连续17d。除空白组外,第15天起各组小鼠每日灌胃30min后于低压氧舱中模拟高海拔环境进行低氧暴露,连续3d。第18天出舱后取小鼠新鲜粪便抹片观察菌群的变化,HE染色观察肠组织的病理形态学改变,免疫组化法检测肠组织中缺氧诱导因子1α(HIF-1α)蛋白的表达变化。结果模型组小鼠肠道球菌及革兰阴性菌百分比(分别为65.2%±2.4%、56.7%±3.3%)明显高于空白组(分别为24.7%±1.2%、23.2%±1.5%,P<0.05),小肠和结肠肠黏膜坏死水肿病理评分(分别为3.10±0.99、3.30±0.67)及炎性细胞计数(分别为15.93±3.30、16.40±3.97/HP)明显高于空白组(分别为0.70±0.67、0.80±0.78、4.07±2.12、4.28±2.16/HP,P<0.05),肠组织HIF-1α表达水平明显高于空白组(P<0.05)。与模型组相比,黄芪百合颗粒中、高剂量组肠道球菌百分比(分别为46.7%±2.0%、32.0%±2.6%)及革兰阴性菌百分比(分别为34.2%±1.6%、28.0%±2.8%)明显下降(P<0.05),小肠和结肠肠黏膜坏死水肿病理评分(小肠分别为2.30±1.33、2.10±0.94,结肠分别为2.50±1.08、1.90±0.99)明显降低(P<0.05),炎性细胞计数(小肠分别为13.26±2.34、10.93±3.67/HP,结肠分别为14.40±2.02、11.33±2.96/HP)明显降低(P<0.05),肠组织HIF-1α的表达水平明显增高(P<0.01)。结论黄芪百合颗粒制剂对高原低氧环境下小鼠肠道微生态及肠黏膜屏障具有一定保护作用。
Objective To investigate the protective effect of Astragalus-Lily Granules on intestinal mucosa and intestinal flora homeostasis in mice under high altitude hypoxia condition. Methods We put mice into high altitude hypoxia cabin to establish high altitude hypoxia model mice. Sixty Kunming mice were randomly divided into control group, model group, Astragalus- Lily particles （ALP） low, medium and high dose groups [1.75, 3.5, 7g/（kg.d）] respectively. After three days of routine feeding, the ALP mice received drug by intragastric administration, once a day for continuous 17 days, control group and model group were given double distilled water in same volume. From the 15th day, all the mice but control group were exposed to simulated high altitude hypoxia condition for 3 days in a high altitude hypoxia cabin after they were ravaged for half an hour daily. By the 18th day, the fresh mouse feces were collected and smeared to observe the changes ofmicroflora. The pathological changes of intestinal tissues were observed by HE staining and the expression of HIF-1α protein in intestines was detected by immunohistochemistry. Results The enterococci and gram negative bacteria showed a higher proportion （65.2% ±2.4% and 56.7% ± 3.3%, respectively） in the model group compared with the control group （24.7% ±1.2%, 23.2% ± 1.5%, respectively, P〈0.05）. The pathological score of intestinal mucosal necrosis and edema （3.10± 0.99, 3.30 ± 0.67 respectively） and inflammatory cell count （15.93 ± 3.30, 16.40 ± 3.97/ HP respectively） was higher compared with the model group （0.70 ±0.67, 0.80 ± 0.78; 4.07 ± 2.12, 4.28± 2.16/HP respectively; P〈0.05）. HIF-lot expression increased significantly compared with the model group （P〈0.05）. The enterococci （46.7% ± 2.0%, 32.0% ± 2.6% respectively） and gram negative bacteria rate （34.2% ±1.6% ,38.0% ± 2.8% respectively） in the ALP medium and high dose groups were lower compared with the model group （24.7%± 1.2%, 23.2% ± 1.5% respectively, P〈0.05）. The pathological score of intestinal mucosal necrosis and edema in small intestine （2.30 ± 1.33, 2.10± 0.94 respectively） and colon （2.50±1.08, 1.90±0.99） were lower than those of model group and inflammatory cell count （small intestine 13.26 ± 2.34, 10.93 ± 3.67/HP, colon 14.40 ±2.02, 11.33 ± 2.96/HP, respectively） were lower than those of the model group （P〈0.05）, and the HIF-1α expression in the intestinal tissues increased significantly compared with the model group （P〈0.01）. Conclusion ALP has certain protective effect on intestinal mucosa and microecology of mice under high altitude hypoxia condition.