文献类型：期刊文献

中文题名：藏药湿生扁蕾提取物对人结肠癌SW480细胞增殖和周期的影响

英文题名：Influence of Tibetan ShiSheng BianLei Extract on the Proliferation and Cycle of Human Colon Cancer SW480 Cells

作者：张艳霞[1,2];景明[1,2];蔺兴遥[1,2];杨雅丽[1];郭红云[3]

第一作者：张艳霞

机构：[1]甘肃中医学院,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室;[3]甘肃省医学科学研究院

第一机构：甘肃中医药大学

年份：2014

卷号：27

期号：9

起止页码：50

中文期刊名：西部中医药

外文期刊名：Western Journal of Traditional Chinese Medicine

收录：CSTPCD

语种：中文

中文关键词：结肠癌;SW480细胞;增殖;湿生扁蕾

外文关键词：colon cancer; SW480 cells; proliferation; ShiSheng BianLei

摘要：目的:探讨藏药湿生扁蕾提取物(GPM)对人结肠癌细胞SW480增殖和周期的作用机制。方法:采用MTT比色法测定GPM对SW480细胞体外细胞毒活性,采用流式细胞仪检测细胞周期变化。结果:GPM对结肠癌SW480细胞的增殖具有抑制作用,且这种抑制作用具有明显的量效关系;用GPM处理后,SW480细胞表现出明显的G1期周期阻滞。结论:GPM能够抑制人结肠癌细胞SW480细胞的生长,并可改变细胞周期分布。
Objective： To find out the mechanism of Tibetan ShiSheng BianLei [Gentianopsis paludosa （Hook. f.）Ma] （GPM） extract on the proliferation and cycle of human colon cancer SW480 cells. Methods： Cytotoxicity effects of GPM to human colon cancer SW480 cells were investigated by MTT chromatometry in vitro, and the changes of cell cycles were detected by flow cytometry. Results： GPM could inhibit the proliferatin of colon cancer SW480 cells, and the inhibition presented obvious dose-effect relationship; after dealt with GPM, SW480 cells indicated remarkable cycle arrest at G1 phase. Conclusion： GPM could inhibit the growth of human colon cancer SW480 cells and change the distribution of cell cycles.