文献类型：期刊文献

英文题名：Diversity of Hepatitis E Viruses in Rats in Yunnan Province and the Inner Mongolia Autonomous Region of China

作者：Li, Li-Li[1,2,3];Ma, Xiao-Hua[4];Nan, Xiao-Wei[5];Wang, Jing-Lin[6];Zhao, Jing[3,7];Sun, Xiao-Man[1,2,3];Li, Jin-Song[1,2,3];Zheng, Gui-Sen[7];Duan, Zhao-Jun[1,2,3]

第一作者：Li, Li-Li

通信作者：Duan, ZJ[1];Duan, ZJ[2];Duan, ZJ[3];Zheng, GS[4]

机构：[1]Natl Key Lab Intelligent Tracking & Forecasting In, Beijing 102206, Peoples R China;[2]NHC Key Lab Med Virol & Viral Dis, Beijing 102206, Peoples R China;[3]China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China;[4]GANSU Prov Ctr Dis Control & Prevent, Lanzhou 730000, Peoples R China;[5]Inner Mongolia Autonomous Reg Acad Prevent Med, Hohhot 010080, Peoples R China;[6]Yunnan Anim Sci & Vet Inst, Yunnan Trop & Subtrop Anim Viral Dis Lab, Kunming 650224, Peoples R China;[7]Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou 730000, Peoples R China

第一机构：Natl Key Lab Intelligent Tracking & Forecasting In, Beijing 102206, Peoples R China

通信机构：[1]corresponding author), Natl Key Lab Intelligent Tracking & Forecasting In, Beijing 102206, Peoples R China;[2]corresponding author), NHC Key Lab Med Virol & Viral Dis, Beijing 102206, Peoples R China;[3]corresponding author), China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China;[4]corresponding author), Gansu Univ Chinese Med, Sch Publ Hlth, Lanzhou 730000, Peoples R China.|[10735e9d5e7087247e71b]甘肃中医药大学公共卫生学院;[10735]甘肃中医药大学;

年份：2025

卷号：17

期号：4

外文期刊名：VIRUSES-BASEL

收录：;Scopus(收录号：2-s2.0-105003665291);WOS:【SCI-EXPANDED(收录号:WOS:001475479800001)】；

基金：This research was funded by National Natural Science Foundation of China [No. 82402622] and National Key Research and Development Program of China [No. 2021YFC2301000]. The APC was funded by the first funder.

语种：英文

外文关键词：rat hepatitis E virus (HEV); Hepeviridae; genetic diversity; complete genome; rodent

摘要：Hepatitis E virus (HEV) is one of the most common pathogens causing acute hepatitis. Rat HEV, a member of the genus Rocahepevirus, infects mainly rat but can also cause human zoonotic infection. A survey of the virome of rats via next-generation sequencing (NGS) was performed in Yunnan Province and Inner Mongolia in China. Further screening of rat HEV was conducted by nested PCR. The complete genome of six representative strains were obtained by NGS and RT-PCR. The virome analysis revealed that multiple reads were annotated as Hepeviridae. The screening results showed that HEV was detected in 9.6% (34 of 355) of the rat samples and phylogenetically classified into three lineages. The sequences from Yunnan clustered with Rocahepevirus ratti, named the YnRHEV group, and those from Inner Mongolia were separated into two lineages, named the NmRHEV-1 and NmRHEV-2 groups. Complete sequence analysis showed that YnRHEV had very high sequence identity to a human HEV strain identified in immunosuppressed patients (88.7% to 94.3%), a reminder of the risk of cross-species transmission of rodent HEV. Notably, NmRHEV-1 and the most closely related rat HEV, RtCb-HEV/HeB2014, were divergent from other HEV. The phylogenetic analyses and lower sequence identities of the complete genome suggested the NmRHEV-1 to be a novel putative genus of the subfamily Orthohepevirinae. NmRHEV-2 shared the highest sequence identities (70.6% to 72.0%) with the species Rocahepevirus eothenomi, which may represent a putative novel genotype. This study revealed high genetic diversity of Hepeviridae in rats in China and a potentially zoonotic Rocahepevirus ratti strain.