文献类型：期刊文献

中文题名：藏药湿生扁蕾对IL-6诱导SW480细胞SATA3活化作用的影响

英文题名：Influence of Gentianopsis paludosa Ma on the expression of SATA3 phosphorylation induced by IL-6 in cell line SW480

作者：王雅莉[1];景明[1,2];杨雅丽[1];张艳霞[1];高娜[1];赵慧巧[1];刘娟娟[1]

第一作者：王雅莉

机构：[1]甘肃中医学院,甘肃兰州730000;[2]甘肃省中药药理与毒理学重点实验室,甘肃兰州730000

第一机构：甘肃中医药大学

年份：2014

卷号：31

期号：1

起止页码：10

中文期刊名：甘肃中医学院学报

外文期刊名：Journal of Gansu College of Traditional Chinese Medicine

基金：国家自然科学基金项目(81360522)

语种：中文

中文关键词：湿生扁蕾;SW480细胞;STAT3通路

外文关键词：Gentianopsis paludosa Ma; SW480 cell; STAT3

摘要：目的探讨藏药湿生扁蕾对白细胞介素-6(IL-6)诱导SW480细胞信号转录和转录激活因子(STAT3)活化作用的影响。方法选用SW480细胞为实验对象,以不同质量浓度(0,250,500,1 000μg/mL)的湿生扁蕾干预,采用Bio-plex悬液芯片法检测SW480细胞STAT3磷酸化的水平。结果不同质量浓度的湿生扁蕾均有抑制SW480细胞STAT3活化的作用,与对照组比较差异有统计意义(P<0.05),并呈质量浓度-剂量依赖关系。结论湿生扁蕾抗结肠癌的作用与其抑制IL-6介导的STAT3信号通路的活化有关。
Objective To observe the effect of Gentianopsis paludosa Ma on the expression of SATA3 phosphorylation induced by IL-6 in cell line SW480. Methods SW480 K562 cells were treated at different dosages （0, 250, 500, and 1000 μg/mL） of Gentianopsis Paludosa Ma, respectively. The expression of STAT3 phosphorylation was determined by the way of bio-plex liquid chip. Results Compared with the control group, STAT3 phosphorylation was decreased significantly in SW480 cells treated with different dosages of gentianopsis paludosa ma in a time and dose dependent manner. Conclusion Gentianopsis paludosa Ma has the effect on SW480 cells may be related to the inhibition of STAT3 phosphorylation mediated induced by IL-6.