文献类型：期刊文献

中文题名：红芪多糖对糖尿病大鼠视网膜血小板反应蛋白-1和血小板源性生长因子-B表达的影响

英文题名：Effects of Hedysari Polysaccharide on Expressions of TSP-1 and PDGF-B in Retina of Diabetic Rats

作者：张花治[1];金智生[1];刘莹[1];颉瑞萍[2];赵建梅[1];高妍[2]

第一作者：张花治

机构：[1]甘肃中医药大学,甘肃兰州730000;[2]甘肃中医药大学附属医院,甘肃兰州730020

第一机构：甘肃中医药大学

年份：2017

卷号：24

期号：3

起止页码：38

中文期刊名：中国中医药信息杂志

外文期刊名：Chinese Journal of Information on Traditional Chinese Medicine

收录：CSTPCD;;CSCD:【CSCD_E2017_2018】；

基金：国家自然科学基金地区基金(81360538);甘肃省青年科技基金计划(145RJYA297)

语种：中文

中文关键词：糖尿病;视网膜;红芪多糖;血小板反应蛋白-1;血小板源性生长因子-B;大鼠

外文关键词：diabetes mellitus; retina; Hedysari Polysaccharide; TSP-1; PDGF-B; rats

摘要：目的观察红芪多糖(HPS)对糖尿病大鼠视网膜血小板反应蛋白-1(TSP-1)和血小板源性生长因子-B(PDGF-B)表达的影响,探讨其对糖尿病视网膜病变的保护作用及可能机制。方法采用链脲佐菌素腹腔注射建立糖尿病模型。雄性Wistar大鼠随机分为模型组、多贝斯组和HPS高、中、低剂量组,另设正常组,每组10只。各给药组给予相应药物灌胃,模型组和正常组给予等量生理盐水灌胃,1次/d,连续8周。q RT-PCR和免疫组化检测TSP-1和PDGF-B m RNA和蛋白表达;HE染色镜下观察视网膜的结构。结果模型组视网膜各层结构清晰、完整,但外核层疏松变薄、排列紊乱,神经节细胞数量稍减少;各给药组较模型组明显好转。与正常组比较,模型组视网膜TSP-1 m RNA和蛋白表达明显降低(P<0.01),PDGF-B m RNA和蛋白表达明显升高(P<0.01);与模型组比较,各给药组TSP-1 m RNA和蛋白表达明显升高(P<0.05,P<0.01),PDGF-B m RNA和蛋白表达明显降低(P<0.01);HPS高剂量组与其余给药组比较,TSP-1和PDGF-B m RNA和蛋白表达差异有统计学意义(P<0.05,P<0.01)。结论 HPS可能通过升高糖尿病大鼠视网膜组织TSP-1的表达和降低PDGF-B的表达来阻遏糖尿病视网膜病变进程中新生血管生成及增殖,从而起到保护视网膜的作用。
Objective To observe the effects of Hedysari Polysaccharide(HPS)on the expressions of TSP-1andPDGF-B in the retina of diabetic rats;To discuss the protective effect and possible mechanism on diabetic retinopathy.Methods The diabetic model was established by intraperitoneal injection of streptozotocin.50male SPF Wistar ratswere randomly divided into5groups:model group,calcium dobesilate group,and HPS high-,medium-,and low-dosegroup,extra10rats were set as the normal group,10rats in each group.Each administration group was given relevantmedicine for gavage,while model group and normal control group were given same amount NS for gavage,once aday for8weeks.The mRNA and protein expression of TSP-1and PDGF-B were detected by qRT-PCR andimmunohistochemistry.The retinal structure was observed by HE staining.Results HE staining showed that eachlayer of the retina of the model group was clear and complete,but the outer nucleus layer became looser,thinner andmore disorderly,and the number of ganglion cells decreased slightly;the administration groups were improvedmarkedly compared with the model group.Compared with the normal control group,the mRNA level and proteinexpression of retina TSP-1on the model group dramatically dropped(P<0.01),and those of PDGF-B strikinglyincreased(P<0.01);Compared with the model group,the mRNA level and protein expression of retina TSP-1on all administration groups rose(P<0.05,P<0.01),and those of PDGF-B went down(P<0.01);Compared with all otheradministration groups,there was statistical significance in the mRNA level and protein expression of retina TSP-1andPDGF-B on HPS high-dose group(P<0.05,P<0.01).Conclusion HPS may prevent the angiogenesis and proliferationin diabetic retinopathy process through adjusting the content of TSP-1and PDGF-B in retina of diabetic rats so as toprotect the retina.