文献类型：期刊文献

中文题名：黄管秦艽中总裂环烯醚萜苷对白细胞介素-1β诱导下大鼠软骨细胞的影响

英文题名：Effect of Gentiana officinalis from Total Secoiridoid Glucoside on Interleukin-1β (IL-1β)-Induced Rat Chondrocytes

作者：李希斌[1];叶娟[1];赵磊[1];夏鹏飞[1];彭雪晶[1];余晓晖[1]

第一作者：李希斌

机构：[1]甘肃中医学院、甘肃省高校中藏药化学与质量研究省级重点实验室,兰州730000

第一机构：甘肃中医药大学科研实验中心（甘肃省中医药标准化技术委员会秘书处）

年份：2014

卷号：49

期号：13

起止页码：1121

中文期刊名：中国药学杂志

外文期刊名：Chinese Pharmaceutical Journal

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；PubMed;

基金：甘肃省高等学校基本科研业务费资助项目(2011-6);甘肃省兰州市城关区科技发展计划项目(BH2012-059)

语种：中文

中文关键词：总裂环烯醚萜苷;软骨细胞;Ⅱ型胶原;前列腺素E2;黄管秦艽

外文关键词：total secoiridoid glucoside ; chondrocytes ; collagen type Ⅱ ; PGE2 ; Gentiana officinalis

摘要：目的通过考查甘肃习用药材黄管秦艽栽培种和野生种中总裂环烯醚萜苷对白细胞介素-1β(IL-1β)诱导的大鼠软骨细胞的影响,探讨栽培种和野生种黄管秦艽中总裂环烯醚萜苷治疗骨关节炎(osteoarthritis,OA)的可行性。方法取大鼠关节软骨细胞第4代,将第4代软骨细胞分为4组:空白组(A组)软骨细胞用完全培养基培养;白细胞介素-1β诱导组(B组)软骨细胞以10ng·mL-1重组大鼠白细胞介素-1β培养24 h;白细胞介素-1β和栽培种黄管秦艽总裂环烯醚萜苷组(C组:分为C1组50μg·mL-1;C2组200μg·mL-1;C3组400μg·mL-1)软骨细胞以10 ng·mL-1重组大鼠白细胞介素-1β和栽培种黄管秦艽总裂环烯醚萜苷(以下称栽培组)培养24 h;白细胞介素-1β和野生种黄管秦艽总裂环烯醚萜苷组(D组:分为D1组50μg·mL-1;D2组100μg·mL-1;D3组200μg·mL-1)软骨细胞以10 ng·mL-1重组大鼠白细胞介素-1β和野生种黄管秦艽总裂环烯醚萜苷(以下称野生组)培养24 h。培养后取各组细胞进行免疫细胞化学染色,收集细胞培养液,检测其上清液中表达前列腺素E2的情况。结果四甲基偶氮唑蓝法结果显示,栽培组(50、200、400μg·mL-1)对软骨细胞作用24 h时无毒性作用,野生组(50、100、200μg·mL-1)对软骨细胞作用24 h时无毒性作用。免疫细胞化学染色显示,栽培组和野生组与诱导组相比Ⅱ型胶原阳性表达明显增强,但弱于空白组,并且随着质量浓度的增大Ⅱ型胶原阳性表达逐渐增强,差异有统计学意义(P<0.05)。酶联免疫法结果显示,栽培组和野生组与诱导组相比前列腺素E2含量明显降低,但高于空白组,并且随着质量浓度的增大前列腺素E2含量越低,差异有统计学意义(P<0.05)。结论栽培种和野生种黄管秦艽中的总裂环烯醚萜苷均对炎症软骨细胞具有一定的保护作用。
OBJECTIVE To investigate the feasibility of cultivated and wild total secoiridoid glucoside in treating osteoarthritis （OA） by observing the effects of total secoiridoid glucoside on intedeukin 1β （ IL-1β ） induced chondroeytes of rat in vitro. METH- ODS Take the four passage rat chondrocytes and divided into 4 groups ： the chondrocytes were cultured with rats complete medium in group A, with 10 ng· mL-1 recombinant rat IL-1β 24 h in group B, with 10 ng· mL-1 recombinant rat IL-1β and cultivated total secoiridoid glucoside 24 h in group C （ different concentrations of cultivated total secoiridoid glucoside is 50,200,400 μg·mL-1 ） , with 10 ng · mL-1 recombinant rat IL-1β and wild total secoiridoid glucoside 24 h in group D（ different concentrations of seed total secoiri- doid glueoside is 50, 100, 200 μg·mL-1 ）. Immunocytoehemistry and Elisa were performed to determine the expression levels of collagen type II and PGE2. RESULTS An MTT assay indicated that 50, 200, 400 μg·mL-1 cultivated total secoiridoid glucoside showed no significant toxicity to chondrocytes（P 〉0. 05） at 24 h; 50, 100, 200 μg·mL-1 wild total secoiridoid glucoside showed no significant toxicity to chondrocytes（P 〉 0. 05 ） at 24 h. Immunocytochemistry showed that the expression of collagen Type Ⅱ in group C and group D obviously increased compared with group B, but weak in group A, and positive expression gradually enhanced with the in- crease of concentration（P 〈0. 05）. ELISA results showed that the production of PGE2 in group C and group D significantly decreased compared with group B, but higher than that of group A, and with the increase of concentration, the lower the production of PGE2 （ P 〈 0. 05 ）. CONCLUSION Cultivated and wild total secoiridoid glucoside can protect rat chondrocyte from OA induced by IL-1β.