文献类型：期刊文献

英文题名：Synthesis, Antioxidant, and Antihypoxia Activities of 6,7,8,4′-Tetrahydroxyisoflavone and 6,7,8,3′,4′-Pentahydroxyisoflavone

作者：Tan, Hongqiang[1,2];Shao, Jin[3];Zhang, Jie[1,2];Ma, Huiping[3];Jing, Linlin[1]

第一作者：Tan, Hongqiang

通信作者：Jing, LL[1]

机构：[1]Hosp Xian Jiaotong Univ, Ctr Brain Sci, Xian, Shaanxi, Peoples R China;[2]Gansu Univ Chinese Med, Coll Pharm, Lanzhou, Gansu, Peoples R China;[3]940th Hosp Joint Logist Support Force PLA, Dept Pharm, Lanzhou, Gansu, Peoples R China

第一机构：Hosp Xian Jiaotong Univ, Ctr Brain Sci, Xian, Shaanxi, Peoples R China

通信机构：[1]corresponding author), Xi An Jiao Tong Univ, Ctr Brain Sci, Affiliated Hosp 1, 277 Yanta West Rd, Xian 710061, Shanxi, Peoples R China.

年份：2022

卷号：17

期号：9

外文期刊名：NATURAL PRODUCT COMMUNICATIONS

收录：;Scopus(收录号：2-s2.0-85138721517);WOS:【SCI-EXPANDED(收录号:WOS:000855607900001)】；

基金：The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded by the National Natural Science Foundation of China (Grant No. 81872796, 81202458, 81571847) and the Logistics Research Program of PLA (Grant No. CWH17J010).

语种：英文

外文关键词：formononetin; hydroxydaidzein; synthesis; antioxidant activity; antihypoxia activity

摘要：In the present study, 6,8-dihydroxydaidzein (6,8-DHD or 6,7,8,4 '-tetrahydroxyisoflavone) and 6,8,3 '-trihydroxydaidzein (6,8,3 '-THD or 6,7,8,3 ',4 '-pentahydroxyisoflavone) were synthesized via a facile and efficient way using commercially available formononetin as starting material. Their structures were confirmed using spectroscopic analyses (infrared, nuclear magnetic resonance, and mass spectrometry). The purity was checked by ultra-high performance liquid chromatography. Their antioxidant activities were evaluated via 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay and reducing power assay using ascorbic acid (vitamin C) as a reference compound. The antihypoxia capacity was determined by a hypoxia injury model in PC12 cells. Our study revealed that 6,8-DHD and 6,8,3 '-THD exhibited higher antioxidant activities than that of vitamin C and could protect PC12 cells against hypoxia-induced damage. These results indicate that 6,8-DHD and 6,8,3 '-THD are excellent antioxidant agents and could be used for alleviating injury induced by hypoxia.